Use of an Enrichment Broth Improves Detection of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae in Clinical Stool Samples

Nathalie Jazmati; Rebecca Hein; Axel Hamprecht

doi:10.1128/JCM.02926-15

Use of an Enrichment Broth Improves Detection of Extended-Spectrum-Beta-Lactamase-Producing Enterobacteriaceae in Clinical Stool Samples

J Clin Microbiol. 2016 Feb;54(2):467-70. doi: 10.1128/JCM.02926-15. Epub 2015 Nov 25.

Authors

Nathalie Jazmati¹, Rebecca Hein², Axel Hamprecht³

Affiliations

¹ Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany [email protected].
² Institute of Medical Statistics, Informatics and Epidemiology, University of Cologne, Cologne, Germany.
³ Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany German Centre for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany.

Abstract

This study evaluated the impact of preenrichment on the detection of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) in clinical stool samples. ESBL-E were detected in 41 of 343 patients (12.0%). As 31.7% of the ESBL-E carriers were identified by preenrichment, only this additional diagnostic step significantly improved the detection of ESBL-E.

MeSH terms

Bacterial Load
Culture Media
Enterobacteriaceae / drug effects
Enterobacteriaceae / enzymology*
Enterobacteriaceae / genetics
Enterobacteriaceae / isolation & purification*
Enterobacteriaceae Infections / diagnosis*
Enterobacteriaceae Infections / microbiology*
Feces / microbiology*
Humans
Microbial Sensitivity Tests
Multiplex Polymerase Chain Reaction / methods
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
beta-Lactamases / biosynthesis*
beta-Lactamases / genetics

Substances

Culture Media
beta-Lactamases