Behavioral characterization of CD36 knockout mice with SHIRPA primary screen

Behav Brain Res. 2016 Feb 15:299:90-6. doi: 10.1016/j.bbr.2015.11.027. Epub 2015 Nov 25.

Abstract

CD36 is a member of the class B scavenger receptor family of cell surface proteins, which plays a major role in fatty acid, glucose and lipid metabolism. Besides, CD36 functions as a microglial surface receptor for amyloid beta peptide. Regarding this, we suggest CD36 might also contribute to neuropsychiatric disease. The aim of this study was to achieve a behavioral phenotype of CD36 knockout (CD36(-/-)) mice. We characterized the behavior of CD36(-/-) mice and C57BL/6J mice by subjecting them to a series of tests, which include SHIRPA primary behavioral screen test, 1% sucrose preference test, elevated plus-maze test, open-field test and forced swimming test. The results showed that CD36(-/-) mice traversed more squares, emitted more defecation, exhibited higher tail elevation and had more aggressive behaviors than C57BL/6J mice. The CD36(-/-) mice spent more time and traveled longer distance in periphery zone in the open-field test. Meanwhile, the numbers that CD36(-/-) mice entered in the open arms of elevated plus-maze were reduced. These findings suggest that CD36(-/-) mice present an anxious phenotype and might be involved in neuropsychiatric disorders.

Keywords: Anxiety; Behavioral phenotype; CD36 knockout mice; SHIRPA primary screen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid beta-Peptides
  • Animals
  • Anxiety / genetics
  • Anxiety / psychology
  • Behavior, Animal / physiology*
  • CD36 Antigens / deficiency*
  • CD36 Antigens / genetics
  • Exploratory Behavior / physiology*
  • Female
  • Male
  • Maze Learning / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Motor Activity / genetics
  • Motor Activity / physiology
  • Phenotype
  • Swimming

Substances

  • Amyloid beta-Peptides
  • CD36 Antigens