Up-regulation of Proinflammatory Genes and Cytokines Induced by S100A8 in CD8+ T Cells in Lichen Planus

Gabriel Costa de Carvalho; Rosana Domingues; Marcelle Almeida de Sousa Nogueira; Anna C Calvielli Castelo Branco; Kelly C Gomes Manfrere; Naiura Vieira Pereira; Valéria Aoki; Mirian Nacagami Sotto; Alberto J Da Silva Duarte; Maria Notomi Sato

doi:10.2340/00015555-2306

Up-regulation of Proinflammatory Genes and Cytokines Induced by S100A8 in CD8+ T Cells in Lichen Planus

Acta Derm Venereol. 2016 May;96(4):485-9. doi: 10.2340/00015555-2306.

Authors

Gabriel Costa de Carvalho¹, Rosana Domingues, Marcelle Almeida de Sousa Nogueira, Anna C Calvielli Castelo Branco, Kelly C Gomes Manfrere, Naiura Vieira Pereira, Valéria Aoki, Mirian Nacagami Sotto, Alberto J Da Silva Duarte, Maria Notomi Sato

Affiliation

¹ Laboratory of Dermatology and Immunodeficiency (LIM-56), Department of Dermatology, Medical School, University of São Paulo, São Paulo, SP, Brazil.

PMID: 26632637
DOI: 10.2340/00015555-2306

Abstract

Lichen planus (LP) is a chronic inflammatory mucocutaneous disease. The inflammatory status of LP may be related to S100A8 (myeloid-related protein 8; MRP8) activation of cytotoxic cells. The aims of this study were to evaluate S100A8 expression in skin lesions and the in vitro effects of S100A8 on CD8+ T cells and natural killer (NK) cells in LP. Increased levels of S100A8/S100A9 were detected in the skin lesions as well as in the sera of subjects with LP. S100A8 expression induced an increased cytotoxic response by peripheral blood CD8+CD107a+ T cells as well as by NK CD56bright cells in patients with LP. Increased expression of interleukin (IL)-1β, tumour necrosis factor (TNF) and IL-6 in the CD8+ T cells of patients with LP was induced by S100A8, in contrast to the control group that produced IL- 10 and interferon (IFN) type I genes. These data suggest that, in individuals with LP, S100A8 may exert distinct immunomodulatory and cytotoxicity functions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD56 Antigen / immunology
CD56 Antigen / metabolism
CD8-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / metabolism*
Calgranulin A / immunology
Calgranulin A / metabolism*
Calgranulin B / immunology
Calgranulin B / metabolism
Case-Control Studies
Cells, Cultured
Cytokines / genetics
Cytokines / immunology
Cytokines / metabolism*
Humans
Inflammation Mediators / immunology
Inflammation Mediators / metabolism*
Killer Cells, Natural / immunology
Killer Cells, Natural / metabolism
Lichen Planus / genetics
Lichen Planus / immunology
Lichen Planus / metabolism*
Lichen Planus / pathology
Lysosomal-Associated Membrane Protein 1 / immunology
Lysosomal-Associated Membrane Protein 1 / metabolism
Signal Transduction
Skin / immunology
Skin / metabolism*
Skin / pathology
Toll-Like Receptor 4 / immunology
Toll-Like Receptor 4 / metabolism
Up-Regulation

Substances

CD56 Antigen
Calgranulin A
Calgranulin B
Cytokines
Inflammation Mediators
Lysosomal-Associated Membrane Protein 1
NCAM1 protein, human
TLR4 protein, human
Toll-Like Receptor 4