Differential expression of two C-type lectins in grass carp Ctenopharyngodon idella and their response to grass carp reovirus

J Fish Biol. 2016 Feb;88(2):787-93. doi: 10.1111/jfb.12815. Epub 2015 Dec 8.

Abstract

The cDNAs of two C-type lectins in grass carp Ctenopharyngodon idella, galactose-binding lectin (galbl) and mannose-binding lectin (mbl), were cloned and analysed in this study. Both of them exhibited the highest expression level in liver, whereas their expression pattern differed in early phase of embryonic development. Following exposure to grass carp reovirus (GCRV), the mRNA expression level of galbl and mbl was significantly up-regulated in liver and intestine.

Keywords: galactose-binding lectin; group III lectins; mannose-binding lectin; maternal expression pattern.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carps / genetics*
  • Carps / virology
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Embryo, Nonmammalian / metabolism
  • Embryonic Development
  • Fish Diseases / genetics*
  • Fish Diseases / virology
  • Fish Proteins / genetics
  • Fish Proteins / metabolism*
  • Galectins / genetics
  • Galectins / metabolism*
  • Gene Expression Regulation, Developmental
  • Intestinal Mucosa / metabolism
  • Liver / metabolism
  • Mannose-Binding Lectin / genetics
  • Mannose-Binding Lectin / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reoviridae
  • Reoviridae Infections / veterinary*

Substances

  • DNA, Complementary
  • Fish Proteins
  • Galectins
  • Mannose-Binding Lectin
  • RNA, Messenger