High Prevalence of SXT/R391-Related Integrative and Conjugative Elements Carrying blaCMY-2 in Proteus mirabilis Isolates from Gulls in the South of France

Salim Aberkane; Fabrice Compain; Dominique Decré; Chloé Dupont; Chrislène Laurens; Marion Vittecoq; Alix Pantel; Jérôme Solassol; Christian Carrière; François Renaud; Nathalie Brieu; Jean-Philippe Lavigne; Nicolas Bouzinbi; Abdoul-Salam Ouédraogo; Hélène Jean-Pierre; Sylvain Godreuil

doi:10.1128/AAC.01654-15

High Prevalence of SXT/R391-Related Integrative and Conjugative Elements Carrying blaCMY-2 in Proteus mirabilis Isolates from Gulls in the South of France

Antimicrob Agents Chemother. 2015 Dec 7;60(2):1148-52. doi: 10.1128/AAC.01654-15. Print 2016 Feb.

Authors

Salim Aberkane¹, Fabrice Compain², Dominique Decré³, Chloé Dupont⁴, Chrislène Laurens⁵, Marion Vittecoq⁶, Alix Pantel⁷, Jérôme Solassol⁸, Christian Carrière⁹, François Renaud¹⁰, Nathalie Brieu¹¹, Jean-Philippe Lavigne⁷, Nicolas Bouzinbi¹², Abdoul-Salam Ouédraogo¹³, Hélène Jean-Pierre⁵, Sylvain Godreuil⁹

Affiliations

¹ Centre Hospitalier Régional Universitaire (CHRU) de Montpellier, Département de Bactériologie-Virologie, Montpellier, France Université Montpellier 1, Montpellier, France INSERM U 1058, Infection by HIV and by Agents with Mucocutaneous Tropism: From Pathogenesis to Prevention, Montpellier, France [email protected].
² Sorbonne University, UPMC Université Paris 06, CR7, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, CIMI, Team E13, Paris, France.
³ Sorbonne University, UPMC Université Paris 06, CR7, CIMI, Team E13 (Bacteriology), Paris, France INSERM U1135, CIMI, Team E13, Paris, France AP-HP, Microbiology, St. Antoine Hospital, Paris, France.
⁴ Université de Montpellier, UMR 5569, Équipe Pathogènes Hydriques Santé Environnements, Montpellier, France.
⁵ Centre Hospitalier Régional Universitaire (CHRU) de Montpellier, Département de Bactériologie-Virologie, Montpellier, France.
⁶ Centre de Recherche de la Tour du Valat, Arles, France Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle, UMR (IRD/CNRS/UM) 5290, Montpellier, France.
⁷ Institut National de la Santé et de la Recherche Médicale, U1047, Université de Montpellier 1, Nîmes, France Department of Microbiology, CHU Carémeau, Nîmes, France.
⁸ Université Montpellier 1, Montpellier, France Department of Biopathology, CHRU, Montpellier, France Department of Clinical Oncoproteomic, Montpellier Cancer Institute, Montpellier, France.
⁹ Centre Hospitalier Régional Universitaire (CHRU) de Montpellier, Département de Bactériologie-Virologie, Montpellier, France Université Montpellier 1, Montpellier, France INSERM U 1058, Infection by HIV and by Agents with Mucocutaneous Tropism: From Pathogenesis to Prevention, Montpellier, France.
¹⁰ Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle, UMR (IRD/CNRS/UM) 5290, Montpellier, France.
¹¹ CH du Pays d'Aix, Laboratoire de Diagnostic Biologique des Maladies Infectieuses et d'Hygiène, Aix-en-Provence, France.
¹² Centre Hospitalier Régional Universitaire (CHRU) de Montpellier, Département de Bactériologie-Virologie, Montpellier, France Université Montpellier 1, Montpellier, France INSERM U 1058, Infection by HIV and by Agents with Mucocutaneous Tropism: From Pathogenesis to Prevention, Montpellier, France Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution et Contrôle, UMR (IRD/CNRS/UM) 5290, Montpellier, France.
¹³ Université Montpellier 1, Montpellier, France INSERM U 1058, Infection by HIV and by Agents with Mucocutaneous Tropism: From Pathogenesis to Prevention, Montpellier, France.

Abstract

The genetic structures involved in the dissemination of blaCMY-2 carried by Proteus mirabilis isolates recovered from different gull species in the South of France were characterized and compared to clinical isolates. blaCMY-2 was identified in P. mirabilis isolates from 27/93 yellow-legged gulls and from 37/65 slender-billed gulls. It was carried by a conjugative SXT/R391-like integrative and conjugative element (ICE) in all avian strains and in 3/7 human strains. Two clinical isolates had the same genetic background as six avian isolates.

MeSH terms

Animals
Charadriiformes / microbiology*
Chromosome Mapping
Chromosomes, Bacterial
Conjugation, Genetic*
Feces / microbiology
France
Humans
Prevalence
Proteus mirabilis / genetics*
Proteus mirabilis / isolation & purification
beta-Lactamases / genetics*

Substances

beta-Lactamases