Hair-bundle proteomes of avian and mammalian inner-ear utricles

Sci Data. 2015 Dec 8:2:150074. doi: 10.1038/sdata.2015.74.

Abstract

Examination of multiple proteomics datasets within or between species increases the reliability of protein identification. We report here proteomes of inner-ear hair bundles from three species (chick, mouse, and rat), which were collected on LTQ or LTQ Velos ion-trap mass spectrometers; the constituent proteins were quantified using MS2 intensities, which are the summed intensities of all peptide fragmentation spectra matched to a protein. The data are available via ProteomeXchange with identifiers PXD002410 (chick LTQ), PXD002414 (chick Velos), PXD002415 (mouse Velos), and PXD002416 (rat LTQ). The two chick bundle datasets compared favourably to a third, already-described chick bundle dataset, which was quantified using MS1 peak intensities, the summed intensities of peptides identified by high-resolution mass spectrometry (PXD000104; updated analysis in PXD002445). The mouse bundle dataset described here was comparable to a different mouse bundle dataset quantified using MS1 intensities (PXD002167). These six datasets will be useful for identifying the core proteome of vestibular hair bundles.

Publication types

  • Dataset
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Chickens
  • Hair Cells, Vestibular* / metabolism
  • Mice
  • Proteome*
  • Proteomics
  • Rats
  • Saccule and Utricle* / metabolism
  • Species Specificity

Substances

  • Proteome