Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

Gerald Larrouy-Maumus; Abigail Clements; Alain Filloux; Ronan R McCarthy; Serge Mostowy

doi:10.1016/j.mimet.2015.12.004

Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

J Microbiol Methods. 2016 Jan:120:68-71. doi: 10.1016/j.mimet.2015.12.004. Epub 2015 Dec 3.

Authors

Gerald Larrouy-Maumus¹, Abigail Clements², Alain Filloux², Ronan R McCarthy², Serge Mostowy³

Affiliations

¹ MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK. Electronic address: [email protected].
² MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK.
³ MRC Centre for Molecular Bacteriology and Infection, Department of Medicine, Imperial College London, London, UK.

Abstract

The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.

Keywords: Endotoxins; Gram-negative bacteria; Mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods
Endotoxins / analysis
Endotoxins / chemistry
Gram-Negative Bacteria / chemistry*
Gram-Negative Bacteria / isolation & purification
Lipid A / analysis
Lipid A / chemistry*
Sensitivity and Specificity
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / instrumentation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Endotoxins
Lipid A

Abstract

Publication types

MeSH terms

Substances

Grants and funding