Suppression of NYVAC Infection in HeLa Cells Requires RNase L but Is Independent of Protein Kinase R Activity

J Virol. 2015 Dec 9;90(4):2135-41. doi: 10.1128/JVI.02576-15. Print 2016 Feb 15.

Abstract

Protein kinase R (PKR) and RNase L are host cell components that function to contain viral spread after infections. In this study, we analyzed the role of both proteins in the abortive infection of human HeLa cells with the poxvirus strain NYVAC, for which an inhibition of viral A27L and B5R gene expression is described. Specifically, the translation of these viral genes is independent of PKR activation, but their expression is dependent on the RNase L activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Endoribonucleases / metabolism*
  • Epithelial Cells / immunology*
  • HeLa Cells
  • Host-Pathogen Interactions*
  • Humans
  • Membrane Glycoproteins / antagonists & inhibitors*
  • Protein Biosynthesis
  • Vaccinia virus / immunology*
  • Viral Envelope Proteins / antagonists & inhibitors*
  • Viral Proteins / antagonists & inhibitors*
  • eIF-2 Kinase / metabolism*

Substances

  • A27L protein, Orthopoxvirus
  • Membrane Glycoproteins
  • Viral Envelope Proteins
  • Viral Proteins
  • 42kDa protein, Vaccinia virus
  • eIF-2 Kinase
  • Endoribonucleases
  • 2-5A-dependent ribonuclease