Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes

Methods Mol Biol. 2016:1433:119-25. doi: 10.1007/7651_2015_295.

Abstract

MHCI-peptide tetramer staining is an important technique in order to identify antigen-specific T cells within a heterogeneous cell population. The reagents may be used to isolate antigen-specific T cells and can help identify their role in disease. Here, we describe how to make tetramer from peptide:MHC monomers together with a protocol for staining antigen-specific cell populations with advice on generating a complementary antibody phenotyping panel.

Keywords: Antigen-specific; Flow cytometry; MHCI-peptide tetramer staining; T cell receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • Diabetes Mellitus, Type 1 / immunology*
  • Disease Models, Animal*
  • Flow Cytometry / methods*
  • Histocompatibility Antigens Class I / immunology*
  • Islets of Langerhans / immunology*
  • Mice
  • Mice, Inbred NOD
  • Mice, Transgenic
  • Staining and Labeling / methods*

Substances

  • Histocompatibility Antigens Class I