Refining the continuum of CFTR-associated disorders in the era of newborn screening

Clin Genet. 2016 May;89(5):539-49. doi: 10.1111/cge.12711. Epub 2016 Jan 20.

Abstract

Clinical heterogeneity in cystic fibrosis (CF) often causes diagnostic uncertainty in infants without symptoms and in older patients with milder phenotypes. We performed a cross-sectional evaluation of a comprehensive set of clinical and laboratory descriptors in a physician-defined cohort (N = 376; Children's Hospital of Wisconsin and the American Family Children's Hospital CF centers in Milwaukee and Madison, WI, USA) to determine the robustness of categorizing CF (N = 300), cystic fibrosis transmembrane conductance regulator (CFTR)-related disorder (N = 19), and CFTR-related (CRMS) metabolic syndrome (N = 57) according to current consensus guidelines. Outcome measures included patient demographics, clinical measures, sweat chloride levels, CFTR genotype, age at diagnosis, airway microbiology, pancreatic function, infection, and nutritional status. The CF cohort had a significantly higher median sweat chloride level (105 mmol/l) than CFTR-related disorder patients (43 mmol/l) and CFTR-related metabolic syndrome patients (35 mmol/l; p ≤ 0.001). Patient groups significantly differed in pancreatic sufficiency, immunoreactive trypsinogen levels, sweat chloride values, genotype, and positive Pseudomonas aeruginosa cultures (p ≤ 0.001). An automated classification algorithm using recursive partitioning demonstrated concordance between physician diagnoses and consensus guidelines. Our analysis suggests that integrating clinical information with sweat chloride levels, CFTR genotype, and pancreatic sufficiency provides a context for continued longitudinal monitoring of patients for personalized and effective treatment.

Keywords: CFTR phenotype-genotype correlation; CFTR-opathies; cystic fibrosis; cystic fibrosis metabolic syndrome; cystic fibrosis-related disorder.

MeSH terms

  • Adolescent
  • Child
  • Chlorides / metabolism
  • Cohort Studies
  • Cross-Sectional Studies
  • Cystic Fibrosis / classification
  • Cystic Fibrosis / diagnosis
  • Cystic Fibrosis / genetics*
  • Cystic Fibrosis Transmembrane Conductance Regulator / genetics*
  • Female
  • Genetic Testing / methods*
  • Genotype
  • Hospitals, Pediatric
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Mutation*
  • Neonatal Screening / methods*
  • Pancreas / physiology
  • Pancreas / physiopathology
  • Pseudomonas aeruginosa / isolation & purification
  • Pseudomonas aeruginosa / physiology
  • Sweat / chemistry
  • Sweat / microbiology

Substances

  • Chlorides
  • Cystic Fibrosis Transmembrane Conductance Regulator