Impact of Variations in Test Method Parameters on In Vitro Activity of Surotomycin against Clostridium difficile and Surotomycin Quality Control Limits for Broth Microdilution and Agar Dilution Susceptibility Testing

J Clin Microbiol. 2016 Mar;54(3):749-53. doi: 10.1128/JCM.02881-15. Epub 2015 Dec 16.

Abstract

Test parameter variations were evaluated for their effects on surotomycin MICs. Calcium concentration was the only variable that influenced MICs; therefore, 50 μg/ml (standard for lipopeptide testing) is recommended. Quality control ranges for Clostridium difficile (0.12 to 1 μg/ml) and Eggerthella lenta (broth, 1 to 4 μg/ml; agar, 1 to 8 μg/ml) were approved by the Clinical and Laboratory Standards Institute based on these data.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Clostridioides difficile / drug effects*
  • Humans
  • Lipopeptides / pharmacology*
  • Microbial Sensitivity Tests / methods*
  • Microbial Sensitivity Tests / standards*
  • Peptides, Cyclic / pharmacology*
  • Quality Control
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Anti-Bacterial Agents
  • CB-183,315
  • Lipopeptides
  • Peptides, Cyclic