Self-Assembled Wound Dressings Silence MMP-9 and Improve Diabetic Wound Healing In Vivo

Adv Mater. 2016 Mar 2;28(9):1809-17. doi: 10.1002/adma.201503565. Epub 2015 Dec 23.

Abstract

The direct local delivery of short interfering RNA (siRNA) into target tissues presents a real solution to several complex medical conditions that today lack efficacious therapies. The development of an ultrathin polymer coating is described to sustain the delivery of siRNA for up to 2 weeks in vitro and in vivo. This technology successfully reduces the expression of MMP-9 within the wounds of diabetic mice, significantly accelerating the wound healing process and improving the quality of tissue formed.

Keywords: controlled local delivery; diabetic wound healing; layer-by-layer; polymer coatings; siRNA delivery.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bandages*
  • Diabetes Mellitus, Experimental / genetics
  • Diabetes Mellitus, Experimental / metabolism
  • Diabetes Mellitus, Experimental / physiopathology*
  • Gene Knockdown Techniques
  • Gene Silencing*
  • Matrix Metalloproteinase 9 / deficiency*
  • Matrix Metalloproteinase 9 / genetics*
  • Matrix Metalloproteinase 9 / metabolism
  • Mice
  • NIH 3T3 Cells
  • Proteolysis
  • RNA, Small Interfering / genetics*
  • Wound Healing / genetics*

Substances

  • RNA, Small Interfering
  • Matrix Metalloproteinase 9