PON-1 Activity and Plasma 8-Isoprostane Concentration in Patients with Angiographically Proven Coronary Artery Disease

Oxid Med Cell Longev. 2015:2015:5136937. doi: 10.1155/2016/5136937. Epub 2015 Nov 30.

Abstract

The aim of the study was to estimate association of the extent of angiographically proven coronary artery disease (CAD) with plasma 8-isoprostane F2 (8-iso-PGF2α) levels as a reliable marker of lipid peroxidation and serum activity of paraoxonase-1, which demonstrates the ability to protect against lipid oxidation. The study included 105 patients with angiographically documented CAD (CAD+) and 45 patients with negative results of coronary angiography (CAD-). Compared to the control group CAD+ patients were characterized by increased 8-iso-PGF2α levels (P = 0.007) and reduced activity of PON-1 towards paraoxon (PONase, P = 0.002) and phenyl acetate (AREase, P = 0.037). Univariate correlation analysis indicated that 8-iso-PGF2α concentrations were positively associated with the severity of CAD as evaluated by the Gensini score (R = 0.41, P < 0.001) while PONase activity (R = -0.26, P < 0.05) and AREase activity (R = -0.23, P < 0.05) were inversely correlated with CAD severity. PONase activity and 8-iso-PGF2α concentration remained independent determinant of atherosclerosis severity in multiple linear regression after adjusting for age, gender, smoking habits, hypertension, type 2 diabetes, statin therapy, and HDL-C and TAG concentration (β coefficients -0.267; P < 0.05 and 0.368; P < 0.001, resp.). The results suggest that PON-1 activity and 8-iso-PGF2α concentration are associated with the presence and extent of coronary stenosis and may be considered additional markers of coronary artery disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aryldialkylphosphatase / metabolism*
  • Carboxylic Ester Hydrolases / metabolism
  • Cholesterol, HDL / blood
  • Coronary Angiography
  • Coronary Artery Disease / diagnostic imaging
  • Coronary Artery Disease / metabolism
  • Coronary Artery Disease / pathology*
  • Dinoprost / analogs & derivatives*
  • Dinoprost / blood
  • Dinoprost / chemistry
  • Dinoprost / metabolism
  • Female
  • Humans
  • Linear Models
  • Male
  • Middle Aged
  • Phenotype
  • Severity of Illness Index

Substances

  • Cholesterol, HDL
  • 8-epi-prostaglandin F2alpha
  • Dinoprost
  • Carboxylic Ester Hydrolases
  • arylesterase
  • Aryldialkylphosphatase
  • PON1 protein, human