The complete mitochondrial genome of Lacerta bilineata and comparison with its closely related congener L. Viridis

Sree Rohit Kolora; Rui Faria; Anne Weigert; Stefan Schaffer; Annegret Grimm; Klaus Henle; Abdullah H Sahyoun; Peter F Stadler; Katja Nowick; Christoph Bleidorn; Martin Schlegel

doi:10.3109/19401736.2015.1111349

The complete mitochondrial genome of Lacerta bilineata and comparison with its closely related congener L. Viridis

Mitochondrial DNA A DNA Mapp Seq Anal. 2017 Jan;28(1):116-118. doi: 10.3109/19401736.2015.1111349. Epub 2015 Dec 28.

Authors

Sree Rohit Kolora^{1

2

3}, Rui Faria^{4

5}, Anne Weigert^{2

6}, Stefan Schaffer², Annegret Grimm⁷, Klaus Henle⁷, Abdullah H Sahyoun³, Peter F Stadler^{3

8

9

10

11

12

13}, Katja Nowick^{3

8

14}, Christoph Bleidorn^{1

2}, Martin Schlegel^{1

2}

Affiliations

¹ a German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig , Leipzig , Germany.
² b Molecular Evolution and Systematics of Animals, Institute of Biology, University of Leipzig , Leipzig , Germany.
³ c Bioinformatics Group, Department of Computer Science, and Interdisciplinary Center for Bioinformatics, Universität Leipzig , Leipzig , Germany.
⁴ d CIBIO, Centro De Investigacßao Em Biodiversidade E Recursos Geneticos, InBio, Laboratorio Associado, Universidade Do Porto , Campus Agrário De Vairão , Vairão , Portugal.
⁵ e Institute of Evolutionary Biology (Universitat Pompeu Fabra-CSIC) , PRBB, Barcelona , Catalonia , Spain.
⁶ f Max Planck Institute for Evolutionary Anthropology , Deutscher Platz 6 , Leipzig , Germany.
⁷ g Department of Conservation Biology , UFZ - Helmholtz Center for Environmental Research , Leipzig , Germany.
⁸ h Paul-Flechsig-Institute for Brain Research, University of Leipzig , Leipzig , Germany.
⁹ i Max-Planck-Institute for Mathematics in the Sciences , Leipzig , Germany.
¹⁰ j Fraunhofer Institut Für Zelltherapie Und Immunologie , Leipzig , Germany.
¹¹ k Department of Theoretical Chemistry , University of Vienna , Wien , Austria.
¹² l Center for non-Coding RNA In Technology and Health, University of Copenhagen , Frederiksberg , Denmark.
¹³ m Santa Fe Institute , Santa Fe , NM , USA , and.
¹⁴ n TFome Research Group, Bioinformatics Group, Department of Computer Science, Interdisciplinary Center of Bioinformatics, University of Leipzig , Leipzig , Germany.

PMID: 26709540
DOI: 10.3109/19401736.2015.1111349

Abstract

We sequenced the mitochondrial genome of the Western green lizard (Lacerta bilineata) using Illumina technology and additional Sanger sequencing. The assembled 17 086 bp mitogenome had a GC content of 40.32% and consisted of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region (CR), with a gene order identical to the chordate consensus. In addition, we re-sequenced the mitogenome of the closely related Eastern green lizard L. viridis using the same techniques as for L. bilineata. The mitogenomes of L. bilineata and L. viridis showed a sequence identity of 94.4% and 99.9%, respectively, relative to the previously published L. viridis mitogenome. The phylogenetic reconstruction based on 17 Lacertinae mitogenomes using Anolis carolinensis as the outgroup supported L. bilineata and its sister species L. viridis as distinct lineages.

Keywords: Complete mitochondrial genome; Illumina sequencing; Lacerta bilineata; Lacerta viridis; Lacertinae; phylogeny.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Composition
Base Sequence
DNA, Mitochondrial
Female
Gene Order
Genes, Mitochondrial*
Genome Size
Genome, Mitochondrial*
Genomics
Lizards / genetics*
Phylogeny*
Sequence Analysis, DNA

Substances

DNA, Mitochondrial