The Fusarium toxin zearalenone and deoxynivalenol affect murine splenic antioxidant functions, interferon levels, and T-cell subsets

Z H Ren; H D Deng; Y C Wang; J L Deng; Z C Zuo; Y Wang; X Peng; H M Cui; J Fang; S M Yu; L H Shen; Y C Hu

doi:10.1016/j.etap.2015.12.007

The Fusarium toxin zearalenone and deoxynivalenol affect murine splenic antioxidant functions, interferon levels, and T-cell subsets

Environ Toxicol Pharmacol. 2016 Jan:41:195-200. doi: 10.1016/j.etap.2015.12.007. Epub 2015 Dec 17.

Authors

Z H Ren¹, H D Deng², Y C Wang³, J L Deng⁴, Z C Zuo⁵, Y Wang⁶, X Peng⁷, H M Cui⁸, J Fang⁹, S M Yu¹⁰, L H Shen¹¹, Y C Hu¹²

Affiliations

¹ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
² College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
³ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China; School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China. Electronic address: [email protected].
⁴ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
⁵ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
⁶ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
⁷ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
⁸ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
⁹ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
¹⁰ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
¹¹ College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].
¹² College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: [email protected].

PMID: 26722803
DOI: 10.1016/j.etap.2015.12.007

Abstract

This study aimed to evaluate the effects of the Fusarium toxin zearalenone (ZEA) and deoxynivalenol (DON) on splenic antioxidant functions, IFN levels, and T-cell subsets in mice. Herein, 360 mice were assigned to nine groups for a 12-day study. Mice were administered an intraperitoneal injection for 4 consecutive days with different concentrations of ZEA alone, DON alone, or ZEA+DON. Spleen and blood samples were collected on days 0, 3, 5, 8, and 12. Mice in each of the experimental groups showed dysreglated splenic antioxidant functions, IFN levels, and T-cell subset frequencies, suggesting that the immune system had been affected. The ZEA+DON-treated groups, especially the group that received a higher concentration of ZEA+DON (Group D2Z2), showed more obvious effects on the dysregulation of splenic antioxidant functions, IFN levels, and T-cell subsets. This finding suggested that DON and ZEA exerted synergistic effects.

Keywords: Antioxidant functions; Deoxynivalenol; Interferon; Mice; T-cell subsets; Zearalenone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Drug Synergism
Fusarium / metabolism
Injections, Intraperitoneal
Interferons / metabolism*
Malondialdehyde / metabolism*
Mice
Mycotoxins / toxicity
Spleen / drug effects*
Spleen / enzymology
T-Lymphocyte Subsets / drug effects*
T-Lymphocyte Subsets / immunology
Trichothecenes / toxicity*
Zearalenone / toxicity*

Substances

Mycotoxins
Trichothecenes
Malondialdehyde
Zearalenone
Interferons
deoxynivalenol