High intensity focused ultrasound as a tool for tissue engineering: Application to cartilage

Adam B Nover; Gary Y Hou; Yang Han; Shutao Wang; Grace D O'Connell; Gerard A Ateshian; Elisa E Konofagou; Clark T Hung

doi:10.1016/j.medengphy.2015.11.016

High intensity focused ultrasound as a tool for tissue engineering: Application to cartilage

Med Eng Phys. 2016 Feb;38(2):192-8. doi: 10.1016/j.medengphy.2015.11.016. Epub 2015 Dec 24.

Authors

Adam B Nover¹, Gary Y Hou², Yang Han³, Shutao Wang⁴, Grace D O'Connell⁵, Gerard A Ateshian⁶, Elisa E Konofagou⁷, Clark T Hung⁸

Affiliations

¹ Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, NY 10027, USA. Electronic address: [email protected].
² Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, NY 10027, USA. Electronic address: [email protected].
³ Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, NY 10027, USA. Electronic address: [email protected].
⁴ Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, NY 10027, USA. Electronic address: [email protected].
⁵ Department of Mechanical Engineering, University of California, Berkeley, USA. Electronic address: [email protected].
⁶ Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, NY 10027, USA; Department of Mechanical Engineering, Columbia University, 242 S. W. Mudd, 500 West 120th Street, Mail Code: 4703, New York, NY 10027, USA. Electronic address: [email protected].
⁷ Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, NY 10027, USA; Department of Radiology, Columbia University, 630 W. 168th Street, P&S Box 28, New York, NY 10032, USA . Electronic address: [email protected].
⁸ Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, Mail Code: 8904, New York, NY 10027, USA. Electronic address: [email protected].

Abstract

This article promotes the use of High Intensity Focused Ultrasound (HIFU) as a tool for affecting the local properties of tissue engineered constructs in vitro. HIFU is a low cost, non-invasive technique used for eliciting focal thermal elevations at variable depths within tissues. HIFU can be used to denature proteins within constructs, leading to decreased permeability and potentially increased local stiffness. Adverse cell viability effects remain restricted to the affected area. The methods described in this article are explored through the scope of articular cartilage tissue engineering and the fabrication of osteochondral constructs, but may be applied to the engineering of a variety of different tissues.

Keywords: Articular cartilage; High intensity focused ultrasound (HIFU); Osteochondral grafts; Tissue engineering.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Biomimetics*
Cartilage, Articular / cytology*
Cattle
Cell Proliferation
Cell Survival
Feasibility Studies
Temperature
Tissue Engineering / methods*
Tissue Scaffolds
Ultrasonic Waves*

Abstract

Publication types

MeSH terms

Grants and funding