Fe₃O₄@Ag magnetic nanoparticles for microRNA capture and duplex-specific nuclease signal amplification based SERS detection in cancer cells

Yuanfeng Pang; Chongwen Wang; Jing Wang; Zhiwei Sun; Rui Xiao; Shengqi Wang

doi:10.1016/j.bios.2015.12.052

Fe₃O₄@Ag magnetic nanoparticles for microRNA capture and duplex-specific nuclease signal amplification based SERS detection in cancer cells

Biosens Bioelectron. 2016 May 15:79:574-80. doi: 10.1016/j.bios.2015.12.052. Epub 2015 Dec 18.

Authors

Yuanfeng Pang¹, Chongwen Wang², Jing Wang³, Zhiwei Sun⁴, Rui Xiao⁵, Shengqi Wang⁶

Affiliations

¹ Capital Medical University, Department of Toxicology, No. 10 Xitoutiao, You An Men, Beijing 100069, PR China; Beijing Institute of Radiation Medicine, Beijing Key Laboratory of New Molecular Diagnosis Techniques for Infectious Dedication, 27 Taiping Road, Beijing, PR China.
² Beijing Institute of Radiation Medicine, Beijing Key Laboratory of New Molecular Diagnosis Techniques for Infectious Dedication, 27 Taiping Road, Beijing, PR China; College of Life Science &Bio-Engineering, Beijing University of Technology, Beijing 100124, PR China.
³ Beijing Institute of Radiation Medicine, Beijing Key Laboratory of New Molecular Diagnosis Techniques for Infectious Dedication, 27 Taiping Road, Beijing, PR China; School of Pharmaceutical Science, Zhengzhou University, Zhengzhou 450001, PR China.
⁴ Capital Medical University, Department of Toxicology, No. 10 Xitoutiao, You An Men, Beijing 100069, PR China. Electronic address: [email protected].
⁵ Beijing Institute of Radiation Medicine, Beijing Key Laboratory of New Molecular Diagnosis Techniques for Infectious Dedication, 27 Taiping Road, Beijing, PR China. Electronic address: [email protected].
⁶ Beijing Institute of Radiation Medicine, Beijing Key Laboratory of New Molecular Diagnosis Techniques for Infectious Dedication, 27 Taiping Road, Beijing, PR China.

PMID: 26749099
DOI: 10.1016/j.bios.2015.12.052

Abstract

A functionalized Fe3O4@Ag magnetic nanoparticle (NP) biosensor for microRNA (miRNA) capture and ultrasensitive detection in total RNA extract from cancer cells was reported in this paper. Herein, Raman tags-DNA probes modified Fe3O4@Ag NPs were designed both as surface-enhanced Raman scattering (SERS) SERS and duplex-specific nuclease signal amplification (DSNSA) platform. Firstly, target miRNAs were captured to the surface of Fe3O4@Ag NPs through DNA/RNA hybridization. In the presence of endonuclease duplex specific nuclease (DSN), one target miRNA molecule could rehybrid thousands of DNA probes to trigger the signal-amplifying recycling. Base on the superparamagnetic of Fe3O4@Ag NPs, target miRNA let-7b can be captured, concentrated and direct quantified within a PE tube without any PCR preamplification treatment. The detection limit was 0.3fM (15 zeptomole, 50μL), nearly 3 orders of magnitude lower than conventional fluorescence based DSN biosensors for miRNA(∼100fM), even single-base difference between the let-7 family members can be discriminated. The result provides a novel proposal to combine the perfect single-base recognition and signal-amplifying ability of the endonuclease DSN with cost-effective SERS strategy for miRNA point-of-care (POC) clinical diagnostics.

Keywords: Duplex-specific nuclease; Fe(3)O(4)@Ag; MiRNA; Paramagnetic; SERS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
HeLa Cells
Humans
Magnetite Nanoparticles / chemistry*
Magnetite Nanoparticles / ultrastructure
MicroRNAs / analysis*
MicroRNAs / genetics
MicroRNAs / isolation & purification
Neoplasms / genetics
Nucleic Acid Amplification Techniques / methods
Point-of-Care Systems*
Silver / chemistry*
Spectrum Analysis, Raman / methods*

Substances

Magnetite Nanoparticles
MicroRNAs
mirnlet7 microRNA, human
Silver