Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity

Nat Methods. 2016 Mar;13(3):229-232. doi: 10.1038/nmeth.3728. Epub 2016 Jan 11.

Abstract

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • Embryonic Stem Cells / physiology*
  • Epigenesis, Genetic / genetics*
  • High-Throughput Nucleotide Sequencing / methods*
  • Mice
  • Molecular Sequence Data
  • Regulatory Elements, Transcriptional / genetics*
  • Transcription Factors / genetics*

Substances

  • Transcription Factors