A stereospecific solid-phase screening assay for colonies expressing both (R)- and (S)-selective ω-aminotransferases

Philos Trans A Math Phys Eng Sci. 2016 Feb 28;374(2061):20150084. doi: 10.1098/rsta.2015.0084.

Abstract

A novel solid-phase screening assay was developed for colonies expressing both (R)- and (S)-selective ω-aminotransferases. This high-throughput assay can be used to screen rapidly large variant libraries with enhanced substrate selectivity and enantioselectivities.

Keywords: aminotransferases; biocatalysis; directed evolution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification*
  • Bacteria / enzymology*
  • Bacteria / isolation & purification
  • Bacterial Typing Techniques / methods*
  • Biological Assay / methods*
  • High-Throughput Screening Assays / methods*
  • Stereoisomerism
  • Transaminases

Substances

  • Transaminases