The immunological activity of Lycium barbarum polysaccharides liposome in vitro and adjuvanticity against PCV2 in vivo

Ruonan Bo; Sisi Zheng; Jie Xing; Li Luo; Yale Niu; Yee Huang; Zhenguang Liu; Yuanliang Hu; Jiaguo Liu; Yi Wu; Deyun Wang

doi:10.1016/j.ijbiomac.2015.12.089

The immunological activity of Lycium barbarum polysaccharides liposome in vitro and adjuvanticity against PCV2 in vivo

Int J Biol Macromol. 2016 Apr:85:294-301. doi: 10.1016/j.ijbiomac.2015.12.089. Epub 2016 Jan 4.

Authors

Ruonan Bo¹, Sisi Zheng¹, Jie Xing¹, Li Luo¹, Yale Niu¹, Yee Huang¹, Zhenguang Liu¹, Yuanliang Hu¹, Jiaguo Liu¹, Yi Wu¹, Deyun Wang²

Affiliations

¹ Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
² Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: [email protected].

PMID: 26763175
DOI: 10.1016/j.ijbiomac.2015.12.089

Abstract

In previous researches, the results showed that Lycium barbarum polysaccharides (LBP) encapsulated with liposome could enhance the immune activity of LBP. Therefore, the present study was designed to investigate the effects of LBPL on spleen lymphocytes and macrophages of mice in vitro and evaluate the immunological adjuvant activity of PCV2 vaccine in vivo. The results showed that LBPL could significantly promote splenocyte proliferation synergistically with PHA or LPS, increase the ratio of CD4(+) to CD8(+) T cells and promote the cytokine secretion of macrophages; enhance PCV2-specific IgG antibody responses, promote Th1 cytokines (IFN-γ and TNF-a) and Th2 cytokine (IL-4) secretion. The histomorphological observation of spleen demonstrated that LBPL as a vaccine adjuvant also has good improvement and stimulating effect on the immune organ.

Keywords: Immune responses; Immunological activity; Lycium barbarum polysaccharides liposome; Vaccine adjuvant.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage*
Adjuvants, Immunologic / chemistry
Animals
Antibodies, Viral / immunology
Antigens, Surface / metabolism
Antiviral Agents / administration & dosage*
Antiviral Agents / chemistry
Biomarkers
Circoviridae Infections / prevention & control
Circovirus / immunology*
Cytokines / biosynthesis
Drugs, Chinese Herbal / administration & dosage*
Drugs, Chinese Herbal / chemistry
Immunoglobulin G / blood
Immunoglobulin G / immunology
Immunologic Factors / administration & dosage*
Immunologic Factors / chemistry
Immunophenotyping
Liposomes*
Lymphocyte Activation / immunology
Lymphocytes / immunology
Lymphocytes / metabolism
Macrophages / immunology
Macrophages / metabolism
Mice
Particle Size
Phenotype
Spleen / immunology
Viral Vaccines / immunology

Substances

Adjuvants, Immunologic
Antibodies, Viral
Antigens, Surface
Antiviral Agents
Biomarkers
Cytokines
Drugs, Chinese Herbal
Immunoglobulin G
Immunologic Factors
Liposomes
Viral Vaccines
lycium barbarum polysaccharide