The research about microscopic structure of emulsion membrane in O/W emulsion by NMR and its influence to emulsion stability

Yiqiao Xie; Jisheng Chen; Shu Zhang; Kaiyan Fan; Gang Chen; Zerong Zhuang; Mingying Zeng; De Chen; Longgui Lu; Linlin Yang; Fan Yang

doi:10.1016/j.ijpharm.2016.01.032

The research about microscopic structure of emulsion membrane in O/W emulsion by NMR and its influence to emulsion stability

Int J Pharm. 2016 Mar 16;500(1-2):110-9. doi: 10.1016/j.ijpharm.2016.01.032. Epub 2016 Jan 16.

Authors

Yiqiao Xie¹, Jisheng Chen², Shu Zhang³, Kaiyan Fan⁴, Gang Chen⁵, Zerong Zhuang⁶, Mingying Zeng⁷, De Chen⁸, Longgui Lu⁹, Linlin Yang¹⁰, Fan Yang¹¹

Affiliations

¹ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
² Department of Pharmacy, First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
³ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
⁴ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
⁵ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
⁶ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
⁷ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
⁸ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
⁹ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
¹⁰ Guangdong Province Maternity and Child Care Hospital, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].
¹¹ Department of Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: [email protected].

PMID: 26784978
DOI: 10.1016/j.ijpharm.2016.01.032

Abstract

Purpose: This paper discussed the influence of microstructure of emulsion membrane on O/W emulsion stability.

Methods: O/W emulsions were emulsified with equal dosage of egg yolk lecithin and increasing dosage of co-emulsifier (oleic acid or HS15). The average particle size and centrifugal stability constant of emulsion, as well as interfacial tension between oil and water phase were determined. The microstructure of emulsion membrane had been studied by (1)H/(13)C NMR, meanwhile the emulsion droplets were visually presented with TEM and IFM.

Results: With increasing dosage of co-emulsifier, emulsions showed two stable states, under which the signal intensity of characteristic group (orient to lipophilic core) of egg yolk lecithin disappeared in NMR of emulsions, but that (orient to aqueous phase) of co-emulsifiers only had some reduction at the second stable state. At the two stable states, the emulsion membranes were neater in TEM and emulsion droplets were rounder in IFM. Furthermore, the average particle size of emulsions at the second stable state was bigger than that at the first stable state.

Conclusions: Egg yolk lecithin and co-emulsifier respectively arranged into monolayer and bilayer emulsion membrane at the two stable states. The microstructure of emulsion membrane was related to the stability of emulsion.

Keywords: (1)H NMR/(13)C NMR spectroscopy; Emulsion membrane; Microscopic structure; O/W emulsion; Stability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Boron Compounds / chemistry
Carbon-13 Magnetic Resonance Spectroscopy
Emulsifying Agents / chemistry
Emulsions / chemistry*
Heterocyclic Compounds, 3-Ring / chemistry
Lecithins / chemistry
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Oleic Acid / chemistry
Particle Size
Polyethylene Glycols / chemistry
Proton Magnetic Resonance Spectroscopy
Soybean Oil / chemistry
Stearic Acids / chemistry
Water / chemistry

Substances

BODIPY-PC
Boron Compounds
Emulsifying Agents
Emulsions
Heterocyclic Compounds, 3-Ring
Lecithins
Stearic Acids
Water
Oleic Acid
Polyethylene Glycols
Solutol HS 15
Soybean Oil