Pin1At regulates PIN1 polar localization and root gravitropism

Wanyan Xi; Ximing Gong; Qiaoyun Yang; Hao Yu; Yih-Cherng Liou

doi:10.1038/ncomms10430

Pin1At regulates PIN1 polar localization and root gravitropism

Nat Commun. 2016 Jan 21:7:10430. doi: 10.1038/ncomms10430.

Authors

Wanyan Xi¹, Ximing Gong¹, Qiaoyun Yang¹, Hao Yu^{1

2}, Yih-Cherng Liou^{1

3}

Affiliations

¹ Department of Biological Sciences, Faculty of Science, National University of Singapore, Lower Kent Ridge Road, 10 Science Drive 4, Singapore 117543, Singapore.
² Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604, Singapore.
³ Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117573, Singapore.

Abstract

Root gravitropism allows plants to establish root systems and its regulation depends on polar auxin transport mediated by PIN-FORMED (PIN) auxin transporters. PINOID (PID) and PROTEIN PHOSPHATASE 2A (PP2A) act antagonistically on reversible phosphorylation of PINs. This regulates polar PIN distribution and auxin transport. Here we show that a peptidyl-prolyl cis/trans isomerase Pin1At regulates root gravitropism. Downregulation of Pin1At suppresses root agravitropic phenotypes of pp2aa and 35S:PID, while overexpression of Pin1At affects root gravitropic responses and enhances the pp2aa agravitropic phenotype. Pin1At also affects auxin transport and polar localization of PIN1 in stele cells, which is mediated by PID and PP2A. Furthermore, Pin1At catalyses the conformational change of the phosphorylated Ser/Thr-Pro motifs of PIN1. Thus, Pin1At mediates the conformational dynamics of PIN1 and affects PID- and PP2A-mediated regulation of PIN1 polar localization, which correlates with the regulation of root gravitropism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / enzymology
Arabidopsis / genetics
Arabidopsis / growth & development
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Gene Expression Regulation, Plant
Gravitropism*
Membrane Transport Proteins / genetics
Membrane Transport Proteins / metabolism*
NIMA-Interacting Peptidylprolyl Isomerase
Peptidylprolyl Isomerase / genetics
Peptidylprolyl Isomerase / metabolism*
Plant Roots / enzymology
Plant Roots / genetics
Plant Roots / growth & development*
Plant Roots / metabolism
Protein Phosphatase 2 / genetics
Protein Phosphatase 2 / metabolism
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism
Protein Transport

Substances

Arabidopsis Proteins
Membrane Transport Proteins
NIMA-Interacting Peptidylprolyl Isomerase
PIN1 protein, Arabidopsis
PIN1AT protein, Arabidopsis
PINOID protein, Arabidopsis
Protein Serine-Threonine Kinases
Protein Phosphatase 2
Peptidylprolyl Isomerase