Protective effects of ψ taraxasterol 3-O-myristate and arnidiol 3-O-myristate isolated from Calendula officinalis on epithelial intestinal barrier

Fitoterapia. 2016 Mar:109:230-5. doi: 10.1016/j.fitote.2016.01.007. Epub 2016 Jan 11.

Abstract

The triterpene esters ᴪ taraxasterol-3-O-myristate (1) and arnidiol-3-O-myristate (2) were tested for their ability to protect epithelial intestinal barrier in an in vitro model. Their effects on ROS production and on trans-epithelial resistance were investigated on CaCo-2 cell monolayers both in basal and stress-induced conditions. Both compounds were able to modulate the stress damage induced by H2O2 and INFγ+TNFα, showing a potential use as model compounds for the study of new therapeutic agents for intestinal inflammations.

Keywords: CaCo-2 cell monolayers; Calendula officinalis; Intestinal inflammation; ROS; TEER; Triterpene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Caco-2 Cells
  • Calendula / chemistry*
  • Epithelial Cells / drug effects*
  • Flowers / chemistry
  • Humans
  • Hydrogen Peroxide
  • Molecular Structure
  • Myristates / chemistry*
  • Reactive Oxygen Species / metabolism
  • Triterpenes / chemistry*
  • Triterpenes / isolation & purification

Substances

  • Myristates
  • Reactive Oxygen Species
  • Triterpenes
  • arnidiol-3-O-myristate
  • taraxasterol-3-O-myristate
  • Hydrogen Peroxide