Effects of deoxynivalenol on calcium homeostasis of concanavalin A--Stimulated splenic lymphocytes of chickens in vitro

Zhihua Ren; Yachao Wang; Huidan Deng; Youtian Deng; Junliang Deng; Zhicai Zuo; Ya Wang; Xi Peng; Hengmin Cui; Liuhong Shen; Shumin Yu; Suizhong Cao

doi:10.1016/j.etp.2016.01.001

Effects of deoxynivalenol on calcium homeostasis of concanavalin A--Stimulated splenic lymphocytes of chickens in vitro

Exp Toxicol Pathol. 2016 Apr;68(4):241-5. doi: 10.1016/j.etp.2016.01.001. Epub 2016 Jan 19.

Authors

Zhihua Ren¹, Yachao Wang², Huidan Deng³, Youtian Deng⁴, Junliang Deng⁵, Zhicai Zuo⁶, Ya Wang⁷, Xi Peng⁸, Hengmin Cui⁹, Liuhong Shen¹⁰, Shumin Yu¹¹, Suizhong Cao¹²

Affiliations

¹ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
² College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China; School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China. Electronic address: [email protected].
³ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
⁴ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
⁵ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
⁶ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
⁷ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
⁸ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
⁹ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
¹⁰ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
¹¹ College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].
¹² College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province Key Laboratory of Animal Disease & Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an, 625014, China. Electronic address: [email protected].

PMID: 26809658
DOI: 10.1016/j.etp.2016.01.001

Abstract

In this study, the in vitro effects of the treatment of concanavalin A (Con A)--stimulated splenic lymphocytes with DON were examined. Splenic lymphocytes isolated from chickens were stimulated with 12.5 μg/mL Con A and exposed to deoxynivalenol (DON) (0-50 μg/mL) for 48 h. The intracellular calcium concentration ([Ca(2+)]i), pH, calmodulin (CaM) mRNA levels, and Na(+),K(+)-ATPase and Ca(2+)-ATPase activities were detected. With the DON exposure concentrations increased, the [Ca(2+)]i and CaM mRNA levels gradually increased in a dose-dependent manner, and all the evaluated conconcentrations affected ATPase activity to the same extent. There were significant differences (P<0.05 or P<0.01) between the treatment groups and the control group. These results indicate that an imbalance in calcium homeostasis and intracellular acidification are components of DON cytotoxicity in chicken lymphocytes.

Keywords: ATPase; CaM; Calcium homeostasis; Deoxynivalenol; Mycotoxin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium / metabolism*
Calcium-Transporting ATPases / metabolism
Calmodulin / metabolism
Cell Culture Techniques
Cells, Cultured
Chickens / immunology
Chickens / metabolism*
Concanavalin A / pharmacology*
Dose-Response Relationship, Drug
Environmental Pollutants / toxicity*
Homeostasis / drug effects*
Lymphocyte Activation / drug effects
Lymphocyte Activation / immunology
Lymphocytes / drug effects*
Lymphocytes / enzymology
Lymphocytes / metabolism
Real-Time Polymerase Chain Reaction
Sodium-Potassium-Exchanging ATPase / metabolism
Spleen / cytology
Spleen / drug effects*
Spleen / metabolism
Trichothecenes / toxicity*

Substances

Calmodulin
Environmental Pollutants
Trichothecenes
Concanavalin A
Calcium-Transporting ATPases
Sodium-Potassium-Exchanging ATPase
deoxynivalenol
Calcium