Effects of Prednisolone on Disease Progression in Antiretroviral-Untreated HIV Infection: A 2-Year Randomized, Double-Blind Placebo-Controlled Clinical Trial

PLoS One. 2016 Jan 26;11(1):e0146678. doi: 10.1371/journal.pone.0146678. eCollection 2016.

Abstract

Background: HIV-disease progression correlates with immune activation. Here we investigated whether corticosteroid treatment can attenuate HIV disease progression in antiretroviral-untreated patients.

Methods: Double-blind, placebo-controlled randomized clinical trial including 326 HIV-patients in a resource-limited setting in Tanzania (clinicaltrials.gov NCT01299948). Inclusion criteria were a CD4 count above 300 cells/μl, the absence of AIDS-defining symptoms and an ART-naïve therapy status. Study participants received 5 mg prednisolone per day or placebo for 2 years. Primary endpoint was time to progression to an AIDS-defining condition or to a CD4-count below 200 cells/μl.

Results: No significant change in progression towards the primary endpoint was observed in the intent-to-treat (ITT) analysis (19 cases with prednisolone versus 28 cases with placebo, p = 0.1407). In a per-protocol (PP)-analysis, 13 versus 24 study participants progressed to the primary study endpoint (p = 0.0741). Secondary endpoints: Prednisolone-treatment decreased immune activation (sCD14, suPAR, CD38/HLA-DR/CD8+) and increased CD4-counts (+77.42 ± 5.70 cells/μl compared to -37.42 ± 10.77 cells/μl under placebo, p < 0.0001). Treatment with prednisolone was associated with a 3.2-fold increase in HIV viral load (p < 0.0001). In a post-hoc analysis stratifying for sex, females treated with prednisolone progressed significantly slower to the primary study endpoint than females treated with placebo (ITT-analysis: 11 versus 21 cases, p = 0.0567; PP-analysis: 5 versus 18 cases, p = 0.0051): No changes in disease progression were observed in men.

Conclusions: This study could not detect any significant effects of prednisolone on disease progression in antiretroviral-untreated HIV infection within the intent-to-treat population. However, significant effects were observed on CD4 counts, immune activation and HIV viral load. This study contributes to a better understanding of the role of immune activation in the pathogenesis of HIV infection.

Trial registration: ClinicalTrials.gov NCT01299948.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anti-HIV Agents / pharmacology
  • Anti-HIV Agents / therapeutic use*
  • Antiretroviral Therapy, Highly Active
  • CD4 Lymphocyte Count
  • Disease Progression
  • Double-Blind Method
  • Female
  • HIV Infections / drug therapy*
  • HIV Infections / epidemiology
  • Humans
  • Immunologic Factors / pharmacology*
  • Immunologic Factors / therapeutic use
  • Kaplan-Meier Estimate
  • Male
  • Medication Adherence
  • Prednisolone / pharmacology*
  • Prednisolone / therapeutic use
  • Treatment Outcome
  • Viral Load

Substances

  • Anti-HIV Agents
  • Immunologic Factors
  • Prednisolone

Associated data

  • ClinicalTrials.gov/NCT01299948

Grants and funding

This work was supported by the German Leprosy and Tuberculosis Relief Association (http://www.dahw.de/home); Evangelisches Studienwerk e.V. Villigst (http://www.evstudienwerk.de/); Georg Ludwig Rexroth Foundation (http://www.rexroth-stiftung.de/); Deutscher Akademischer Austauschdienst (DAAD) (https://www.daad.de/de/index.html); and the Unibund Würzburg (http://www.unibund.uni-wuerzburg.de/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.