First Characterization of CTX-M-15-Producing Escherichia coli Strains Belonging to Sequence Type (ST) 410, ST224, and ST1284 from Commercial Swine in South America

Ketrin C Silva; Marina Moreno; Carlos Cabrera; Beny Spira; Louise Cerdeira; Nilton Lincopan; Andrea M Moreno

doi:10.1128/AAC.02788-15

First Characterization of CTX-M-15-Producing Escherichia coli Strains Belonging to Sequence Type (ST) 410, ST224, and ST1284 from Commercial Swine in South America

Antimicrob Agents Chemother. 2016 Mar 25;60(4):2505-8. doi: 10.1128/AAC.02788-15. Print 2016 Apr.

Authors

Ketrin C Silva¹, Marina Moreno¹, Carlos Cabrera¹, Beny Spira², Louise Cerdeira³, Nilton Lincopan⁴, Andrea M Moreno⁵

Affiliations

¹ Department of Preventive Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
² Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
³ Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
⁴ Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil [email protected] [email protected].
⁵ Department of Preventive Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil [email protected] [email protected].

Abstract

We report for the first time the isolation of CTX-M-15-producingEscherichia colistrains belonging to sequence type (ST) 410, ST224, and ST1284 in commercial swine in Brazil. TheblaCTX-M-15gene was located on F-::A9::B1 and C1::A9::B1 IncF-type plasmids, surrounded by a new genetic context comprising the IS26insertion sequence truncated with the ISEcp1element upstream ofblaCTX-M-15 These results reveal that commercial swine have become a new reservoir of CTX-M-15-producing bacteria in South America.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Brazil / epidemiology
Cephalosporin Resistance
Cephalosporins / pharmacology
DNA Transposable Elements
Escherichia coli / drug effects
Escherichia coli / enzymology
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology
Escherichia coli Infections / microbiology
Escherichia coli Infections / veterinary*
Gene Expression
Genotype
Meat / microbiology*
Microbial Sensitivity Tests
Plasmids / chemistry*
Plasmids / metabolism
Swine
Swine Diseases / epidemiology*
Swine Diseases / microbiology
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Cephalosporins
DNA Transposable Elements
ceftiofur
beta-lactamase CTX-M-15
beta-Lactamases