Fetuin-A is Associated to Serum Calcium and AHSG T256S Genotype but Not to Coronary Artery Calcification

Biochem Genet. 2016 Jun;54(3):222-231. doi: 10.1007/s10528-016-9714-4. Epub 2016 Jan 29.

Abstract

Vascular calcification has been recently associated to an increased cardiovascular risk and mortality. In few studies, Fetuin-A showed an association to coronary artery calcification (CAC), although the physiopathological mechanism underlying this association has not been fully established yet. Seventy-four patients with one or more cardiovascular risk factor and asymptomatic for coronary vasculopathy were included in the study. CAC was evaluated by Agatston score. Serum Fetuin-A levels were determined by ELISA. Molecular analysis of AHSG T256S gene variant (rs4918) was performed by PCR-RFLP. Serum Fetuin-A was correlated to serum calcium (r = 0,321; P = 0,018), but not to serum phosphorous. Multivariate linear regression analysis confirmed this association and showed that calcium and AHSG genotype were independent predictors of Fetuin-A (P = 0.037, P = 0.014, respectively). In particular, subjects carrying the SS genotype had lower levels of Fetuin-A and calcium (P = 0.037 and P = 0.038, respectively). When we compare subjects with CAC 0-10 with subjects with CAC > 10, we found that only age and male gender (P < 0.001, P = 0.035, respectively), but not Fetuin-A, were associated to CAC. Fetuin-A is not associated to CAC in subjects with low cardiovascular risk profile and asymptomatic for coronary vasculopathy, suggesting that in this setting Fetuin-A, although correlated to serum levels of calcium, could be not involved in mineral deposition on coronary vessels.

Keywords: AHSG; Coronary artery calcification; Coronary artery disease; Fetuin-A; Serum calcium.

MeSH terms

  • Aged
  • Amplified Fragment Length Polymorphism Analysis
  • Calcium / blood*
  • Coronary Vessels / pathology*
  • Female
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Vascular Calcification / blood
  • Vascular Calcification / genetics*
  • alpha-2-HS-Glycoprotein / genetics*
  • alpha-2-HS-Glycoprotein / metabolism

Substances

  • AHSG protein, human
  • alpha-2-HS-Glycoprotein
  • Calcium