[Complementary addressed alkylation of 16s rRNA from Escherichia coli by 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivatives of oligodeoxyribonucleotides. III. Segments of 16s rRNA interacting with the benzylidene derivative of d(pACCTTGTT)rA]

M A Zenkova; G G Karpova; A S Levina

[Complementary addressed alkylation of 16s rRNA from Escherichia coli by 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivatives of oligodeoxyribonucleotides. III. Segments of 16s rRNA interacting with the benzylidene derivative of d(pACCTTGTT)rA]

Mol Biol (Mosk). 1989 Jul-Aug;23(4):1057-66.

[Article in Russian]

Authors

M A Zenkova, G G Karpova, A S Levina

PMID: 2685545

Abstract

16S rRNA chain was cleaved by RNase H within covalent adduct with a 2',3'-0-[4-N-(2-chloroethyl)-N-methylamino]benzylidene derivative of d(pACCTTGTT)rA. It was shown that no less than 50% of cleavage occurs at the 1498-1506 site. The selectivity of alkylation and correspondingly the cleavage by RNase H, at this site practically does not increase when RNA was alkylated to a low extent, and also when a small excess of the reagent in respect to rRNA was used.

MeSH terms

Alkylation
Base Sequence
Electrophoresis, Polyacrylamide Gel
Escherichia coli / metabolism*
Hydrolysis
Molecular Sequence Data
Nitrogen Mustard Compounds / metabolism*
Nucleic Acid Conformation
Oligodeoxyribonucleotides / metabolism*
RNA, Ribosomal / metabolism*
RNA, Ribosomal, 16S / metabolism*

Substances

Nitrogen Mustard Compounds
Oligodeoxyribonucleotides
RNA, Ribosomal
RNA, Ribosomal, 16S