MSRV envelope protein is a potent, endogenous and pathogenic agonist of human toll-like receptor 4: Relevance of GNbAC1 in multiple sclerosis treatment

J Neuroimmunol. 2016 Feb 15:291:29-38. doi: 10.1016/j.jneuroim.2015.12.006. Epub 2015 Dec 11.

Abstract

Multiple sclerosis associated retrovirus envelope protein (MSRV-Env) was repeatedly detected in brain lesions and blood of multiple sclerosis (MS) patients. We performed the first pharmacological characterisation of MSRV-Env on recombinant and native human TLR4. MSRV-Env is a full and highly potent TLR4 agonist of endogenous origin. MSRV-Env induces TLR4-dependent pro-inflammatory stimulation of immune cells in vitro and in vivo, and impairs oligodendrocytes precursor cells differentiation to myelinating oligodendrocytes. MSRV-Env may play a role in chronic inflammation and impaired remyelination in MS. GNbAC1, a selective monoclonal antibody, antagonizes MSRV-Env pathogenic effects and represents an innovative therapeutic approach of MS.

Keywords: GNbAC1; HERV-W; MSRV envelope; Monoclonal antibody; Multiple sclerosis; TLR4.

MeSH terms

  • Animals
  • Antibodies, Monoclonal, Humanized / pharmacology*
  • Brain / pathology
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Cytokines / metabolism
  • Endogenous Retroviruses / genetics
  • Female
  • Gene Products, env / genetics
  • Gene Products, env / metabolism*
  • Humans
  • Immunosuppressive Agents / pharmacology
  • Lipopolysaccharide Receptors / metabolism
  • Lipopolysaccharides / adverse effects
  • Mice
  • Mice, Inbred C57BL
  • Multiple Sclerosis / pathology*
  • Oligodendroglia / drug effects
  • Signal Transduction / drug effects
  • Stem Cells / drug effects
  • Time Factors
  • Toll-Like Receptor 4 / antagonists & inhibitors
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / metabolism*

Substances

  • Antibodies, Monoclonal, Humanized
  • Cytokines
  • Gene Products, env
  • Immunosuppressive Agents
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • TLR4 protein, human
  • Toll-Like Receptor 4
  • temelimab