miR-373 Inhibits Glioma Cell U251 Migration and Invasion by Down-Regulating CD44 and TGFBR2

Cell Mol Neurobiol. 2016 Nov;36(8):1389-1397. doi: 10.1007/s10571-016-0338-3. Epub 2016 Feb 8.

Abstract

Glioblastoma multiforme (GBM) is the most malignant glioma, unveiling the underlying mechanisms of its aggressiveness could promote the discovery of potential targets for effective treatment. MicroRNAs (miRNAs) are important participants in both development and disease, its involvement in cancers has long been recognized. In this study, we investigated the role of miRNA-373 (miR-373) in GBM cell line U251, demonstrated that although miR-373 does not affect cell growth of U251, it inhibits migration and invasion of U251. Forced expression of miR-373 down-regulates the expressions CD44 and TGFBR2, while knockdown of CD44 and TGFBR2 presents the similar phenotype as miR-373 overexpression, suggesting that CD44 and TGFBR2 are functional targets of miR-373, down-regulation of CD44 and TGFBR2 by miR-373 are partly responsible for the migration, and invasion suppressive role of miR-373 in U251.

Keywords: CD44; Glioma; Invasion; Migration; TGFBR2; miRNA-373.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement*
  • Down-Regulation
  • Glioblastoma / genetics
  • Glioblastoma / metabolism*
  • Humans
  • Hyaluronan Receptors / metabolism*
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness*
  • Protein Serine-Threonine Kinases / metabolism*
  • Receptor, Transforming Growth Factor-beta Type II
  • Receptors, Transforming Growth Factor beta / metabolism*

Substances

  • CD44 protein, human
  • Hyaluronan Receptors
  • MIRN373 microRNA, human
  • MicroRNAs
  • Receptors, Transforming Growth Factor beta
  • Protein Serine-Threonine Kinases
  • Receptor, Transforming Growth Factor-beta Type II