Abstract
The effects of concentrated fibroblast-conditioned media were tested to determine whether hepatocyte function can be maintained without direct contact between hepatocytes and fibroblasts. Primary rat hepatocytes cultured with a concentrated conditioned media of NIH-3T3 J2 cell line (final concentration of 55 mg/ml) showed significantly improved survival and functions (albumin and urea) compared to those of control groups. They also showed higher expression levels of mRNA, albumin and tyrosine aminotransferase compared to hepatocyte monoculture. The results suggest that culture with concentrated fibroblast-conditioned media could be an easy method for in vitro maintenance of primary hepatocytes. They also could be contribute to understand and analyze co-culture condition of hepatocyte with stroma cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells / metabolism*
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Albumins / biosynthesis
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Albumins / genetics
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Animals
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Cell Survival
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Cells, Cultured
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Chromatography, Gel
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Chromatography, High Pressure Liquid
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Culture Media, Conditioned / pharmacology*
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Culture Media, Serum-Free / pharmacology
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Female
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Hepatocytes / drug effects*
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Hepatocytes / metabolism
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Mice
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Polymerase Chain Reaction
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Primary Cell Culture / methods*
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Rats
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Rats, Sprague-Dawley
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Stromal Cells
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Urea / metabolism
Substances
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Albumins
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Culture Media, Conditioned
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Culture Media, Serum-Free
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Urea
Grants and funding
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2011-0030075, and 2011-0028845). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.