Expression of functional antibody Fv and Fab fragments in Escherichia coli

Methods Enzymol. 1989:178:497-515. doi: 10.1016/0076-6879(89)78036-8.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Affinity / methods
  • Cloning, Molecular / methods*
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics*
  • Gene Expression
  • Genes, Immunoglobulin
  • Humans
  • Immunoglobulin Fab Fragments / genetics*
  • Immunoglobulin Fab Fragments / isolation & purification
  • Immunoglobulin Fragments*
  • Immunoglobulin Variable Region / genetics*
  • Immunoglobulin Variable Region / isolation & purification
  • Models, Molecular
  • Molecular Weight
  • Plasmids
  • Protein Conformation
  • Recombinant Proteins / genetics*
  • Recombinant Proteins / isolation & purification

Substances

  • Immunoglobulin Fab Fragments
  • Immunoglobulin Fragments
  • Immunoglobulin Variable Region
  • Recombinant Proteins
  • immunoglobulin Fv