Assessment of Expression of Genes Coding GABAA Receptors during Chronic and Acute Intoxication of Laboratory Rats with Ethanol

Bull Exp Biol Med. 2016 Feb;160(4):452-4. doi: 10.1007/s10517-016-3194-8. Epub 2016 Feb 23.

Abstract

Expression of genes encoding the individual subunits of ionotropic GABAA receptor was assessed after acute and chronic intoxication of rats with ethanol. The chronic 1-month-long exposure to ethanol signifi cantly decreased (by 38%) expression of Gabrb1 gene in the hippocampus. Acute exposure to ethanol elevated expression of genes Gabrb1 (by 1.7 times), Gabra1 (by 3.8 times), and Gabra4 (by 6.5 times), although it diminished expression of Gabra2 gene by 1.4 times. In preliminarily alcoholized rats, acute intoxication with ethanol enhanced expression of genes Gabrb1 and Gabra5 by 1.7 and 8.7 times, respectively. There was neither acute nor chronic effect of ethanol on expression of gene Gabra3.

Keywords: GABAA receptors; ethanol; expression; gene.

MeSH terms

  • Alcoholic Intoxication / metabolism
  • Animals
  • Ethanol / adverse effects*
  • Gene Expression / drug effects*
  • Gene Expression Regulation / drug effects*
  • Hippocampus / metabolism
  • Male
  • Rats
  • Receptors, GABA-A / biosynthesis*
  • Receptors, GABA-A / genetics

Substances

  • Gabra1 protein, rat
  • Gabra2 protein, rat
  • Gabra3 protein, rat
  • Gabra4 protein, rat
  • Gabra5 protein, rat
  • Gabrb1 protein, rat
  • Receptors, GABA-A
  • Ethanol