High-resolution microbiota flow cytometry reveals dynamic colitis-associated changes in fecal bacterial composition

Eur J Immunol. 2016 May;46(5):1300-3. doi: 10.1002/eji.201646297.

Abstract

Using high-resolution flow cytometry of bacterial shape (forward scatter) and DNA content (DAPI staining), we detected dramatic differences in the fecal microbiota composition during murine colitis that were validated using 16S rDNA sequencing. This innovative method provides a fast and inexpensive tool to interrogate the microbiota on the single-cell level.

Keywords: Flow cytometry; IBD; Microbiota; Single-cell analysis; T-cell transfer colitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / classification
  • Bacteria / cytology
  • Bacteria / genetics*
  • Bacteria / isolation & purification*
  • Colitis / microbiology*
  • Feces / microbiology*
  • Flow Cytometry / methods*
  • Gastrointestinal Microbiome*
  • Humans
  • Inflammatory Bowel Diseases / microbiology
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics

Substances

  • RNA, Ribosomal, 16S