Effect of Peritoneal Lavage with Clindamycin-Gentamicin Solution on Post-Operative Pain and Analytic Acute-Phase Reactants after Laparoscopic Sleeve Gastrectomy

Surg Infect (Larchmt). 2016 Jun;17(3):357-62. doi: 10.1089/sur.2015.196. Epub 2016 Feb 24.

Abstract

Background: Peritoneal lavage has been proposed to remove bacterial contamination and other materials promoting bacterial proliferation and pro-inflammatory cytokines that may enhance local inflammation. The aims of this study were to evaluate the effects of peritoneal lavage with physiologic saline or an antibiotic solution (clindamycin-gentamicin) on post-operative pain and analytic acute-phase reactants and to determine the microbiologic impact of both irrigations on peritoneal contamination in patients undergoing laparoscopic sleeve gastrectomy (LSG) as a bariatric procedure.

Methods: The patients were randomized into two groups: Those undergoing an intra-abdominal lavage with physiologic saline (Group 1; n = 40) and those undergoing a similar lavage with a gentamicin-clindamycin solution (Group 2; n = 40). Peritoneal contamination, post-operative pain, and analytic acute-phase reactants 24 h after surgery were investigated.

Results: The median pain score 24 h after surgery was 3.5 in Group 1 and 1.5 in Group 2 (p = 0.021). The glucose concentration (mean difference 33.1 mg/dL; p = 0.004), aspartate aminotransferase (AST) concentration (mean difference 41.8 U/L; p = 0.009), alanine aminotransferase (ALT) concentration (mean difference 34.2 U/L; p = 0.029), white blood cell count (mean difference 1930/mm(3); p = 0.029); C-reactive protein concentration (mean difference 27.3 mg/L; p = 0.036), and serum lactic acid concentration (mean difference 0.34 mg/L; p = 0.049) were significantly higher in Group 1. Peritoneal contamination also was significantly higher in Group 1 (17.5% vs 0; p = 0.006).

Conclusions: Intra-peritoneal irrigation with a gentamicin and clindamycin solution in patients undergoing LSG leads to a reduction in post-operative pain and peritoneal contamination, less alteration of acute-phase reactants, lower blood glucose concentration, a smaller increase in liver enzymes, and a shorter hospital stay.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Acute-Phase Proteins / metabolism*
  • Adult
  • Anti-Bacterial Agents / administration & dosage*
  • Anti-Bacterial Agents / therapeutic use
  • Ascitic Fluid / microbiology
  • Biomarkers / blood
  • Clindamycin / administration & dosage*
  • Clindamycin / therapeutic use
  • Female
  • Gastrectomy* / methods
  • Gentamicins / administration & dosage*
  • Gentamicins / therapeutic use
  • Humans
  • Laparoscopy
  • Male
  • Middle Aged
  • Pain, Postoperative / diagnosis
  • Pain, Postoperative / prevention & control*
  • Peritoneal Lavage / methods*
  • Single-Blind Method
  • Solutions
  • Surgical Wound Infection / prevention & control
  • Treatment Outcome

Substances

  • Acute-Phase Proteins
  • Anti-Bacterial Agents
  • Biomarkers
  • Gentamicins
  • Solutions
  • Clindamycin