Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples

Hang Ren; Mingjuan Yang; Guoxia Zhang; Shiwei Liu; Xinhui Wang; Yuehua Ke; Xinying Du; Zhoujia Wang; Liuyu Huang; Chao Liu; Zeliang Chen

doi:10.1016/j.mcp.2016.02.007

Development of a rapid recombinase polymerase amplification assay for detection of Brucella in blood samples

Mol Cell Probes. 2016 Apr;30(2):122-4. doi: 10.1016/j.mcp.2016.02.007. Epub 2016 Feb 18.

Authors

Hang Ren¹, Mingjuan Yang¹, Guoxia Zhang², Shiwei Liu³, Xinhui Wang¹, Yuehua Ke¹, Xinying Du¹, Zhoujia Wang¹, Liuyu Huang¹, Chao Liu¹, Zeliang Chen⁴

Affiliations

¹ Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
² General Hospital of Heilongjiang Land Reclamation Bureau, Harbin, 150088, China.
³ Wangjing Hospital, Academy of Traditional Chinese Medicine, 100102, Beijing, China.
⁴ Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China. Electronic address: [email protected].

PMID: 26911890
DOI: 10.1016/j.mcp.2016.02.007

Abstract

A rapid and sensitive recombinase polymerase amplification (RPA) assay, Bruce-RPA, was developed for detection of Brucella. The assay could detect as few as 3 copies of Brucella per reaction within 20 min. Bruce-RPA represents a candidate point-of-care diagnosis assay for human brucellosis.

Keywords: Brucella; Point-of-care diagnosis; Recombinase polymerase amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood / microbiology
Brucellosis / blood
Brucellosis / diagnosis*
DNA Primers / genetics
Humans
Molecular Diagnostic Techniques / methods
Nucleic Acid Amplification Techniques / methods*
Recombinases / metabolism*
Sensitivity and Specificity

Substances

DNA Primers
Recombinases