Abstract
Different proteases from the culture fluids of recombinant Streptomyces lividans strains were isolated. Several individual proteases were separated and characterized. A chymotrypsin-chylike activity (CLA) was identified that specifically degrades a fusion protein between the alpha-amylase inhibitor from S. tendae (Tendamistat, HOE467) and proinsulin from the monkey Macaca fascicularis. The effective chemical inhibition of the degrading enzyme is demonstrated.
MeSH terms
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Amino Acid Sequence
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Animals
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Bacterial Proteins / metabolism
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Chromatography, Ion Exchange
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Chymotrypsin / antagonists & inhibitors
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Chymotrypsin / metabolism
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Metals / pharmacology
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Molecular Sequence Data
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Peptide Hydrolases / isolation & purification
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Peptide Hydrolases / metabolism*
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Recombinant Fusion Proteins / metabolism
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Streptomyces / enzymology*
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Streptomyces / genetics
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Streptomyces / growth & development
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Time Factors
Substances
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Bacterial Proteins
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Metals
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Recombinant Fusion Proteins
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Peptide Hydrolases
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Chymotrypsin