Smash and DASH with Cas9

Vijay Ramani; Jay Shendure

doi:10.1186/s13059-016-0905-4

Smash and DASH with Cas9

Genome Biol. 2016 Mar 5:17:42. doi: 10.1186/s13059-016-0905-4.

Authors

Vijay Ramani¹, Jay Shendure^{2

3}

Affiliations

¹ Department of Genome Sciences, University of Washington, 3720 15th Ave NE, Seattle, WA, 98195, USA. [email protected].
² Department of Genome Sciences, University of Washington, 3720 15th Ave NE, Seattle, WA, 98195, USA. [email protected].
³ Howard Hughes Medical Institute, 3720 15th Ave NE, Seattle, WA, 98195, USA. [email protected].

Abstract

DeRisi and colleagues present a creative application for Cas9 in vitro, using it to deplete unwanted sequence from DNA libraries. It seems plausible that the in vitro use of CRISPR/Cas9 has unrealized potential to revolutionize the practice of molecular biology well beyond genome editing.

MeSH terms

CRISPR-Cas Systems / genetics
Communicable Diseases / genetics
High-Throughput Nucleotide Sequencing / methods*
Humans
Neoplasms / genetics
Nucleic Acid Hybridization / methods*
RNA / genetics
RNA Editing / genetics
RNA, Mitochondrial
RNA, Ribosomal / genetics
Sequence Analysis, DNA*

Substances

RNA, Mitochondrial
RNA, Ribosomal
RNA