CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering

Daniel J Dickinson; Bob Goldstein

doi:10.1534/genetics.115.182162

CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering

Genetics. 2016 Mar;202(3):885-901. doi: 10.1534/genetics.115.182162.

Authors

Daniel J Dickinson¹, Bob Goldstein²

Affiliations

¹ Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280 [email protected].
² Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280.

Abstract

The advent of genome editing techniques based on the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system has revolutionized research in the biological sciences. CRISPR is quickly becoming an indispensible experimental tool for researchers using genetic model organisms, including the nematode Caenorhabditis elegans. Here, we provide an overview of CRISPR-based strategies for genome editing in C. elegans. We focus on practical considerations for successful genome editing, including a discussion of which strategies are best suited to producing different kinds of targeted genome modifications.

Keywords: CRISPR/Cas9; Caenorhabditis elegans; WormBook; genome editing.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Animals
CRISPR-Cas Systems*
Caenorhabditis elegans / genetics*
DNA Repair
Genetic Engineering / methods*
Mutagenesis, Site-Directed

Abstract

Publication types

MeSH terms

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