Abstract
We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Automation, Laboratory / instrumentation*
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Equipment Design
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Humans
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Influenza A virus / genetics
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Influenza, Human / virology
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Magnetics*
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Nucleic Acids / isolation & purification*
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Pharynx / virology
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RNA, Viral / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction
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Sensitivity and Specificity
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Time Factors