First report of novel genetic array aacA4-blaIMP-25-oxa30-catB3 and identification of novel metallo-β-lactamase gene blaIMP25: A Retrospective Study of antibiotic resistance surveillance on Psuedomonas aeruginosa in Guangzhou of South China, 2003-2007

Guangchao Yu; Wangrong Wen; Brian M Peters; Junyan Liu; Congxiu Ye; Yuchuan Che; Juzhen Liu; Kaiyuan Cao; Zhenbo Xu; Mark E Shirtliff

doi:10.1016/j.micpath.2016.02.021

First report of novel genetic array aacA4-blaIMP-25-oxa30-catB3 and identification of novel metallo-β-lactamase gene blaIMP25: A Retrospective Study of antibiotic resistance surveillance on Psuedomonas aeruginosa in Guangzhou of South China, 2003-2007

Microb Pathog. 2016 Jun:95:62-67. doi: 10.1016/j.micpath.2016.02.021. Epub 2016 Mar 17.

Authors

Guangchao Yu¹, Wangrong Wen¹, Brian M Peters², Junyan Liu³, Congxiu Ye⁴, Yuchuan Che¹, Juzhen Liu¹, Kaiyuan Cao⁵, Zhenbo Xu⁶, Mark E Shirtliff⁷

Affiliations

¹ Clinical Laboratory Center, The First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
² Department of Clinical Pharmacy, College of Pharmacy, University of Tennessee Health Sciences Center, Memphis, TN 38163, USA.
³ College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
⁴ Department of Dermatology and Venerology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
⁵ Department of Microbiology, Zhongshan Medical School of Sun Yat-sen University, Guangzhou 510080, China. Electronic address: [email protected].
⁶ College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA. Electronic address: [email protected].
⁷ Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA.

PMID: 26997650
DOI: 10.1016/j.micpath.2016.02.021

Abstract

Carbapenem, imipenem and meropenem have been broadly prescribed contributing to the global occurrence and prevalence of carbapenem resistance in Psuedomonas aeruginosa, and the associated resistance genotypes remains clinically significant. A retrospective surveillance had been conducted on 499 P. aeruginosa isolates in South China during 2003-2007, including antimicrobial resistance and characterization of MBLs on carbapenem-resistant strains. One hundred and sixty-four out of 499 strains were carbapenem-resistant, with 11, 4 and 5 strains positive for blaIMP-9, blaIMP-25 and blaVIM-2, respectively. Sixteen out of 20 isolates were positive for intI1 and contained identical flanking regions (as indicated in KM384735), and all tested isolates containing the qacE△1-sul1 of the typical 3'-conserved region. A novel blaIMP-25 metallo-β-lactamase and a genetic array of aacA4-blaIMP-25-oxa30-catB3 have been discovered from this retrospective surveillance on antimicrobial resistance of P. aeruginosa.

Keywords: Carbapenem-resistant; Metallo-β-lactamase; P. aeruginosa; aacA4-blaIMP-25-oxa30-catB3; bla(IMP-25).

MeSH terms

Amino Acid Sequence
Anti-Bacterial Agents / pharmacology
Carbapenems / pharmacology
China
Cluster Analysis
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Drug Resistance, Bacterial*
Epidemiological Monitoring
Gene Order
Genes, Bacterial*
Humans
Integrons
Microbial Sensitivity Tests
Molecular Sequence Data
Phylogeny
Pseudomonas Infections / microbiology*
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / genetics
Retrospective Studies
Sequence Alignment
Sequence Analysis, DNA
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Carbapenems
DNA, Bacterial
beta-Lactamases