Anti-Aspergillus immunoglobulin-G testing in serum of hematopoietic stem cell transplant recipients

Transpl Infect Dis. 2016 Jun;18(3):354-60. doi: 10.1111/tid.12529. Epub 2016 May 17.

Abstract

Background: Pulmonary invasive aspergillosis (IA) is a major clinical problem in patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). Acquisition of IA during allo-HSCT by inhalation of spores is the rationale for the widespread use of air filtration systems. Recent data suggest that activation of fungal growth in already colonized patients is a relevant factor, and a recent study found a positive correlation of serum immunoglobulin responses against purified recombinant Aspergillus fumigatus proteins before allo-HSCT with the incidence of IA after allo-HSCT.

Methods: To investigate the clinical utility of this approach, we performed a prospective study. We used a commercially available and standardized assay for detection of anti-Aspergillus immunoglobulin-G (aA-IgG) in serum (Platelia(™) Aspergillus IgG) that has previously demonstrated high sensitivity and specificity.

Results: In a cohort of 104 allo-HSCT recipients, we measured aA-IgG and Aspergillus antigen serum levels before allo-HSCT, and weekly during hospital stay. Overall prevalence of possible, probable, and proven IA during hospital stay was 10%, 6%, and 0%. We found no correlation between aA-IgG levels before allo-HSCT, or after allo-HSCT, and the prevalence of IA during hospital stay. Furthermore, median aA-IgG levels did not differ between patients with history of probable or proven IA, as compared to patients without history of IA.

Conclusions: Taken together, our data argue against the clinical utility of measuring aA-IgG levels for diagnosis or prediction of IA in patients undergoing allo-HSCT.

Keywords: allogeneic; diagnosis; hematopoietic cell transplantation; invasive aspergillosis; invasive fungal infection.

Publication types

  • Observational Study

MeSH terms

  • Adult
  • Aged
  • Antifungal Agents / pharmacology
  • Aspergillus / immunology*
  • Female
  • Hematopoietic Stem Cell Transplantation / adverse effects*
  • Humans
  • Immunoglobulin G / blood*
  • Invasive Pulmonary Aspergillosis / diagnosis*
  • Invasive Pulmonary Aspergillosis / drug therapy
  • Invasive Pulmonary Aspergillosis / epidemiology
  • Invasive Pulmonary Aspergillosis / microbiology
  • Male
  • Middle Aged
  • Prospective Studies
  • Reagent Kits, Diagnostic
  • Young Adult

Substances

  • Antifungal Agents
  • Immunoglobulin G
  • Reagent Kits, Diagnostic