On-off Bodipy chemosensor for recognition of iron(III) ion based on the inner filter effect and its applications in cellular and bacterial imaging

Luminescence. 2016 Dec;31(8):1448-1455. doi: 10.1002/bio.3127. Epub 2016 Mar 22.

Abstract

One strong fluorescent Bodipy-containing derivative was synthesized and characterized using 1 H NMR, electrospray ionization mass spectrometry and elemental analysis. Its electrochemical and photophysical properties were investigated. In addition, the Bodipy derivative could be used as an on-off fluorescent probe for the detection of Fe3+ ions based on the inner filter effect because the absorption band of the Fe3+ ion overlaps the excitation band of Bodipy very well upon irradiation with UV light. Furthermore, the Bodipy-based sensor has obvious advantages including simplicity, rapid response, high selectivity, sensitivity and a detection limit of 1.2 μmol/L, and has been demonstrated in real water samples including tap water, mineral water and water from Lake Tai. Moreover, the fluorescent probe could also be used as a probe for the determination of Fe3+ in cellular and bacterial imaging. Copyright © 2016 John Wiley & Sons, Ltd.

Keywords: Bodipy; cellular imaging, bacterial imaging; fluorescent probe, iron(III).

MeSH terms

  • Bacteria / chemistry
  • Bacteria / ultrastructure
  • Boron Compounds / chemical synthesis
  • Boron Compounds / chemistry*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Ions / analysis
  • Iron / analysis
  • Iron / chemistry*
  • Limit of Detection
  • Magnetic Resonance Spectroscopy
  • Molecular Imaging
  • Staining and Labeling / methods*

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fluorescent Dyes
  • Ions
  • Iron