Meta-Analysis in Gene Expression Studies

Methods Mol Biol. 2016:1418:161-76. doi: 10.1007/978-1-4939-3578-9_8.

Abstract

This chapter introduces methods to synthesize experimental results from independent high-throughput genomic experiments, with a focus on adaptation of traditional methods from systematic review of clinical trials and epidemiological studies. First, it reviews methods for identifying, acquiring, and preparing individual patient data for meta-analysis. It then reviews methodology for synthesizing results across studies and assessing heterogeneity, first through outlining of methods and then through a step-by-step case study in identifying genes associated with survival in high-grade serous ovarian cancer.

Keywords: Biomarkers; Computational molecular biology; Gene expression profiling; Meta-analysis; Microarray analysis; Ovarian neoplasms.

MeSH terms

  • Computational Biology / methods
  • Databases, Genetic
  • Datasets as Topic
  • Gene Expression Profiling / methods*
  • Gene Expression*
  • Humans
  • Meta-Analysis as Topic*
  • Oligonucleotide Array Sequence Analysis / methods