Targeting protein homeostasis in sporadic inclusion body myositis

Sci Transl Med. 2016 Mar 23;8(331):331ra41. doi: 10.1126/scitranslmed.aad4583.

Abstract

Sporadic inclusion body myositis (sIBM) is the commonest severe myopathy in patients more than 50 years of age. Previous therapeutic trials have targeted the inflammatory features of sIBM but all have failed. Because protein dyshomeostasis may also play a role in sIBM, we tested the effects of targeting this feature of the disease. Using rat myoblast cultures, we found that up-regulation of the heat shock response with arimoclomol reduced key pathological markers of sIBM in vitro. Furthermore, in mutant valosin-containing protein (VCP) mice, which develop an inclusion body myopathy, treatment with arimoclomol ameliorated disease pathology and improved muscle function. We therefore evaluated arimoclomol in an investigator-led, randomized, double-blind, placebo-controlled, proof-of-concept trial in sIBM patients and showed that arimoclomol was safe and well tolerated. Although arimoclomol improved some IBM-like pathology in the mutant VCP mouse, we did not see statistically significant evidence of efficacy in the proof-of-concept patient trial.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Amyloid beta-Peptides / metabolism
  • Animals
  • Cell Cycle Proteins / metabolism
  • Cell Survival / drug effects
  • Cells, Cultured
  • Clinical Trials as Topic
  • Endoplasmic Reticulum Stress / drug effects
  • HSP70 Heat-Shock Proteins / metabolism
  • Homeostasis*
  • Humans
  • Hydroxylamines / pharmacology
  • Hydroxylamines / therapeutic use
  • Inflammation Mediators / metabolism
  • Mice
  • Muscle Contraction / drug effects
  • Muscle Strength / drug effects
  • Mutation / genetics
  • Myoblasts / drug effects
  • Myoblasts / metabolism
  • Myoblasts / pathology
  • Myositis, Inclusion Body / metabolism*
  • Myositis, Inclusion Body / pathology
  • Myositis, Inclusion Body / physiopathology
  • Proteins / metabolism*
  • Rats
  • Treatment Outcome
  • Valosin Containing Protein

Substances

  • Amyloid beta-Peptides
  • Cell Cycle Proteins
  • HSP70 Heat-Shock Proteins
  • Hydroxylamines
  • Inflammation Mediators
  • Proteins
  • Adenosine Triphosphatases
  • VCP protein, human
  • Valosin Containing Protein
  • Vcp protein, mouse
  • Vcp protein, rat
  • arimoclomol