Profile of microRNA in Blood Plasma of Healthy Humans

M Yu Skurnikov; Yu A Makarova; E N Knyazev; K A Fomicheva; K M Nyushko; E K Saribekyan; B Ya Alekseev; A D Kaprin

doi:10.1007/s10517-016-3235-3

Profile of microRNA in Blood Plasma of Healthy Humans

Bull Exp Biol Med. 2016 Mar;160(5):632-4. doi: 10.1007/s10517-016-3235-3. Epub 2016 Mar 29.

Authors

M Yu Skurnikov^{1

2}, Yu A Makarova^{3

4}, E N Knyazev⁵, K A Fomicheva³, K M Nyushko³, E K Saribekyan³, B Ya Alekseev³, A D Kaprin³

Affiliations

¹ P. A. Gertsen Moscow Research Oncological Institute, Branch of the Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia. [email protected].
² Bioclinicum Research and Engineering Center, Moscow, Russia. [email protected].
³ P. A. Gertsen Moscow Research Oncological Institute, Branch of the Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
⁴ V. A. Engelhard Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
⁵ Bioclinicum Research and Engineering Center, Moscow, Russia.

PMID: 27021098
DOI: 10.1007/s10517-016-3235-3

Abstract

Analysis of the plasma microRNA profile can be used for the diagnosis of various pathological and physiological conditions. Complete microRNA microprofiling is an extremely important task. Here we used microarray analysis allowing measurement of the expression of 2500 microRNA (MirBase, version 20). About 10% known microRNA were found in the plasma. Most of the detected microRNA (69 microRNA; ~30%) were encoded by mirtrons.

Keywords: blood plasma; healthy volunteers; microRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Genetic Markers
Healthy Volunteers
Humans
Male
MicroRNAs / blood*
Neoplasms / blood*
Neoplasms / diagnosis*
Neoplasms / genetics
Oligonucleotide Array Sequence Analysis

Substances

Genetic Markers
MicroRNAs