A new formula to calculate activity of superoxide dismutase in indirect assays

Anal Biochem. 2016 Jun 15:503:65-7. doi: 10.1016/j.ab.2016.03.014. Epub 2016 Mar 28.

Abstract

To calculate superoxide dismutase (SOD) activity rapidly and accurately by indirect SOD assays, a formula based on the ratio of the catalytic speed of SOD to the reaction speed of the indicator with superoxide anion was deduced. The accuracy of this formula was compared with the conventional formula based on inhibition in five indirect SOD assays. The new formula was validated in nearly the entire SOD activity range, whereas the conventional formula was validated only during inhibition of 40-60%. This formula might also be used for the assays of other enzymes.

Keywords: Cytochrome c; Epinephrine; Hydroxylamine hydrochloride; Nitroblue tetrazolium; SOD; WST.

Publication types

  • Validation Study

MeSH terms

  • Biocatalysis / drug effects
  • Enzyme Assays / methods*
  • Enzyme Inhibitors / pharmacology
  • Hydroxylamine / pharmacology
  • Kinetics
  • Superoxide Dismutase / antagonists & inhibitors
  • Superoxide Dismutase / metabolism*
  • Time Factors

Substances

  • Enzyme Inhibitors
  • Hydroxylamine
  • Superoxide Dismutase