Gene Expression Profiling of Tuberculous Meningitis Co-infected with HIV

Ghantasala S Sameer Kumar; Abhilash K Venugopal; Manoj Kumar Kashyap; Rajesh Raju; Arivusudar Marimuthu; Shyam Mohan Palapetta; Yashwanth Subbanayya; Renu Goel; Ankit Chawla; Jyoti Bajpai Dikshit; Pramila Tata; H C Harsha; Jagadeesha Maharudraiah; Y L Ramachandra; Parthasarathy Satishchandra; T S Keshava Prasad; Akhilesh Pandey; Anita Mahadevan; S K Shankar

doi:10.4172/jpb.1000243

Gene Expression Profiling of Tuberculous Meningitis Co-infected with HIV

J Proteomics Bioinform. 2012 Sep;5(9):235-244. doi: 10.4172/jpb.1000243. Epub 2012 Sep 9.

Authors

Ghantasala S Sameer Kumar¹, Abhilash K Venugopal², Manoj Kumar Kashyap³, Rajesh Raju³, Arivusudar Marimuthu³, Shyam Mohan Palapetta⁴, Yashwanth Subbanayya⁵, Renu Goel¹, Ankit Chawla⁶, Jyoti Bajpai Dikshit⁷, Pramila Tata⁷, H C Harsha³, Jagadeesha Maharudraiah³, Y L Ramachandra⁸, Parthasarathy Satishchandra⁹, T S Keshava Prasad¹⁰, Akhilesh Pandey¹¹, Anita Mahadevan¹², S K Shankar¹²

Affiliations

¹ Institute of Bioinformatics, International Technology Park, Bangalore 560066, Karnataka, India; Department of Biotechnology, Kuvempu University, Shimoga 577451, India.
² Institute of Bioinformatics, International Technology Park, Bangalore 560066, Karnataka, India; Department of Biotechnology, Kuvempu University, Shimoga 577451, India; McKusick-Nathans Institute of Genetic Medicine; Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
³ Institute of Bioinformatics, International Technology Park, Bangalore 560066, Karnataka, India.
⁴ Institute of Bioinformatics, International Technology Park, Bangalore 560066, Karnataka, India; Centre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry, 605014, India.
⁵ Institute of Bioinformatics, International Technology Park, Bangalore 560066, Karnataka, India; Rajiv Gandhi University of Health Sciences, Bangalore 560041, India.
⁶ Armed Forces Medical College, Pune-411040, India.
⁷ Strand Life Sciences, Bangalore 560024, Karnataka, India.
⁸ Department of Biotechnology, Kuvempu University, Shimoga 577451, India.
⁹ Department of Neurology, National Institute of Mental Health and Neurosciences, Bangalore 560029, India.
¹⁰ Institute of Bioinformatics, International Technology Park, Bangalore 560066, Karnataka, India; Centre of Excellence in Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry, 605014, India; Manipal University, Madhav Nagar, Manipal 576104, India.
¹¹ McKusick-Nathans Institute of Genetic Medicine; Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pathology and Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
¹² Department of Neuropathology, National Institute of Mental Health and Neurosciences, Bangalore 560029, India.

Abstract

Tuberculous meningitis (TBM) is a fatal form of Mycobacterium tuberculosis infection of the central nervous system (CNS). The similarities in the clinical and radiological findings in TBM cases with or without HIV make the diagnosis very challenging. Identification of genes, which are differentially expressed in brain tissues of HIV positive and HIV negative TBM patients, would enable better understanding of the molecular aspects of the infection and would also serve as an initial platform to evaluate potential biomarkers. Here, we report the identification of 796 differentially regulated genes in brain tissues of TBM patients co-infected with HIV using oligonucleotide DNA microarrays. We also performed immunohistochemical validation and confirmed the abundance of four gene products-glial fibrillary acidic protein (GFAP), serpin peptidase inhibitor, clade A member 3 (SERPINA3), thymidine phosphorylase (TYMP/ECGF1) and heat shock 70 kDa protein 8 (HSPA8). Our study paves the way for understanding the mechanism of TBM in HIV positive patients and for further validation of potential disease biomarkers.

Keywords: Cerebrospinal fluid; Early diagnosis; GeneSpring; Neurological disorder; Single color labeling.

Abstract

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