Cellular and humoral influenza-specific immune response upon vaccination in patients with common variable immunodeficiency and unclassified antibody deficiency

Vaccine. 2016 May 5;34(21):2417-2423. doi: 10.1016/j.vaccine.2016.03.091. Epub 2016 Apr 5.

Abstract

Background: Immunization against seasonal influenza with inactivated vaccine is recommended for patients with common variable immunodeficiency (CVID). However, humoral vaccine response in CVID patients is frequently impaired and current knowledge on T cell vaccine response in CVID and other patients with antibody deficiency is poor.

Objective: In the present study, we comparatively analyzed the antibody and T cellular immune response of patients with CVID and unclassified antibody deficiency to influenza vaccination in the season 2013-2014.

Methods: Eight patients with CVID, 8 patients with unclassified antibody deficiency and 9 healthy controls were vaccinated with a single dose of non-adjuvanted seasonal influenza vaccine. Before and 3 weeks after the vaccination antibody titers against the strains A/California/7/2009, A/Texas/50/2012, and B/Massachusetts/02/2012 included in the vaccine were measured by hemagglutination inhibition testing. Additionally, vaccine-specific T cell cytokine response was determined by stimulation with the complete vaccine in vitro.

Results: Whereas all healthy controls responded to vaccination with serum antibody titers, only 1/8 CVID patients and 4/8 patients with unclassified antibody deficiency showed a response against at least 1 of the 3 vaccine strains. However, 7/8 of the CVID and 6/8 of the patients with unclassified antibody deficiency had similar frequencies of vaccine-induced IFN-γ, TNF-α and IL-2 producing CD40L(+) T cells as the control group.

Conclusion: Our data suggest that most CVID and unclassified antibody deficiency patients benefit from seasonal influenza vaccination by mounting a cellular response.

Keywords: CVID; Cytokines; Influenza vaccination; T cell response; Unclassified antibody deficiency.

MeSH terms

  • Adult
  • Antibodies, Viral / blood*
  • CD40 Ligand / immunology
  • Common Variable Immunodeficiency / immunology*
  • Female
  • Healthy Volunteers
  • Hemagglutination Inhibition Tests
  • Humans
  • Immunity, Cellular
  • Immunity, Humoral
  • Immunologic Deficiency Syndromes / immunology*
  • Influenza A Virus, H1N1 Subtype / immunology
  • Influenza Vaccines / immunology*
  • Influenza, Human / prevention & control
  • Influenza, Human / virology
  • Interferon-gamma / immunology
  • Interleukin-2 / immunology
  • Male
  • Middle Aged
  • Orthomyxoviridae / immunology*
  • T-Lymphocytes / immunology*
  • Tumor Necrosis Factor-alpha / immunology
  • Vaccination
  • Vaccines, Inactivated / immunology

Substances

  • Antibodies, Viral
  • Influenza Vaccines
  • Interleukin-2
  • Tumor Necrosis Factor-alpha
  • Vaccines, Inactivated
  • CD40 Ligand
  • Interferon-gamma