BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes

Nat Struct Mol Biol. 2016 May;23(5):387-94. doi: 10.1038/nsmb.3204. Epub 2016 Apr 11.

Abstract

The mRNAs stored in oocytes undergo general decay during the maternal-zygotic transition (MZT), and their stability is tightly interconnected with meiotic cell-cycle progression. However, the factors that trigger decay of maternal mRNA and couple this event to oocyte meiotic maturation remain elusive. Here, we identified B-cell translocation gene-4 (BTG4) as an MZT licensing factor in mice. BTG4 bridged CNOT7, a catalytic subunit of the CCR4-NOT deadenylase, to eIF4E, a key translation initiation factor, and facilitated decay of maternal mRNA. Btg4-null females produced morphologically normal oocytes but were infertile, owing to early developmental arrest. The intrinsic MAP kinase cascade in oocytes triggered translation of Btg4 mRNA stored in fully grown oocytes by targeting the 3' untranslated region, thereby coupling CCR4-NOT deadenylase-mediated decay of maternal mRNA with oocyte maturation and fertilization. This is a key step in oocyte cytoplasmic maturation that determines the developmental potential of mammalian embryos.

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Base Sequence
  • Cell Cycle Proteins / physiology*
  • Cells, Cultured
  • Eukaryotic Initiation Factor-4E / physiology
  • Exoribonucleases
  • Female
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Gene Knockout Techniques
  • MAP Kinase Signaling System
  • Male
  • Meiosis
  • Mice, Inbred ICR
  • Mice, Knockout
  • Oocytes / physiology*
  • Proteins / metabolism
  • RNA Stability
  • Repressor Proteins
  • Ribonucleases

Substances

  • 3' Untranslated Regions
  • Btg4 protein, mouse
  • Cell Cycle Proteins
  • Eukaryotic Initiation Factor-4E
  • Proteins
  • Repressor Proteins
  • eIF4E protein, mouse
  • Cnot7 protein, mouse
  • Exoribonucleases
  • Ribonucleases